RP-HPLC on the wet flat buds luteolin and 1,7 - dihydroxy -3,8 - dimethoxy ketone content.docVIP

 PAGE \* MERGEFORMAT 9 RP-HPLC on the wet flat buds luteolin and 1,7 - dihydroxy -3,8 - dimethoxy ketone content Study: Fan Qin Shi Jing Xia Ming Li Jiwen [Abstract]: Objective To establish the wet ingredients in the bud flat luteolin and 1,7 - dihydroxy -3,8 - dimethoxy  determination of ketones. Methods using high performance liquid chromatography (RP- HPLC), column was ZORBAX SB-C18 (4.6 mm × 250 mm, 5 μm), mobile phase was methanol (A) -0.4% phosphoric acid (B) gradient elution, 0 ~ 15 min, A: B = 50 : 50,15 ~ 60 min, A: B = 55:45, detection wavelength 260 nm, flow rate 1 mL /