Salvia miltiorrhiza water-soluble component content of the HPLC method of_0.docVIP

 PAGE \* MERGEFORMAT 9 Salvia miltiorrhiza water-soluble component content of the HPLC method of Of: Liman Ling, Feng Wei Hung, Hong Chen, Liang at Xin, Yang Hongjun, He Xirong, MATERIA [Abstract] Objective To establish a water-soluble components in Radix Salvia Sal B, Danshensu and determination of protocatechuic aldehyde. Methods using high performance liquid chromatography column was Kromosil C18 (4.6 mm × 250 mm, 5 μm), mobile phase Ⅰ: methanol - acetonitrile - formic acid - water (30:10:1:59), mobile phase Ⅱ: methanol - acetic acid - water (20:1:80), flow rate: 1.0 mL / min, detection wavelengths were 286,280 nm, column temperature was 35 ℃. outcome measures 10 different origin of Salvia miltiorrhiza, red acid content of 0.09% B 6.81%, the average recovery was 99.64%, RSD = 1.09%, Danshensu content of 0.16% to 0.68%, the average recovery was 98.94%, RSD = 1.49%, the content of protocatechuic aldehyde in 0.01% and 0.09%, the average recovery was 98.96%, RSD = 1.66 %. Conclusion The method is simple, accurate, reproducible and can be used as standards for the determination of Salvia miltiorrhiza. [Keywords:] Salvia, Sal B, Danshensu, protocatechuic aldehyde, high performance liquid chromatography Keywords:: Salvia miltiorrhiza Bge., Salvianolic acid B, Danshensu, Protocatechuic aldehyde, HPLC Labiatae Salvia Plant Salvia Salvia miltiorrhiza Bge.’s Dried root and rhizome, bitter, slightly cold, the heart, the liver, with blood stasis and pain, promoting blood circulation, the role of pure heart Chufan. For irregular menstruation, The close pass by, Zheng Jia accumulation, abdominal irritation, pain, heat paralysis, sore throat, angina. Salvia is one of the main effective components of Salvia miltiorrhiza water-soluble components, mainly phenolic compounds Sal B, Danshensu and protocatechuic aldehyde. We used HPLC method to establish this determination of 3 components method can be used for SM and Pieces and the drug research and quality control