Scutellaria barbata drug-containing serum on apoptosis of hepatoma H22 cells and mitochondrial membrane potential of.docVIP

 PAGE \* MERGEFORMAT 2 Scutellaria barbata drug-containing serum on apoptosis of hepatoma H22 cells and mitochondrial membrane potential of Authors: DAI Zhi-jun, Wang Xijing, Xue Qian, Zong-positive, Xiao-Xu Liu, Hua-Feng Kang, Guan Hai-Tao, Ma Xiaobin, Ren Hongtao [Abstract] Objective: To explore Scutellaria barbata extract (Scutellaria Barbata extract, ESB)-containing serum on apoptosis rate of liver cancer H22 and mitochondrial membrane potential impact. Methods: In vitro cultured mouse H22 hepatoma cells to ESB high, medium and low-dose drug-containing serum-free culture fluid acting on the H22 cells, and the establishment of blank serum and 5  FU (fluorouracil, 5  Fu) group (positive control group) . Application of tetrazolium salt assay of drugs on cell proliferation; observed under transmission electron microscopy ultrastructural changes of tumor cells; PI tags, flow cytometry cell cycle and cell apoptosis; fluorescent probe rhodamine 123 loading, laser scanning confocal microscope observation of the fluorescence intensity of H22 cells, analysis of mitochondrial membrane potential H22 cell size. Results: ESB-containing serum has some role in inhibiting H22 cell proliferation, high-, medium-dose group 96 h inhibition rates were (47.5 ± 6.4)% and (36.3 ± 4.5)%. Some cells can be seen under transmission electron microscope showed the typical morphological changes of apoptosis. Flow cytometry showed that, ESB, the high-dose group, significant induction of apoptosis, the apoptotic rates were (3.15 ± 1.12)% and (7.83 ± 2.25)%, as compared with the control group the difference was statistical significance (P