Saturated fatty acids on pancreatic -cell apoptosis and cell cycle progression.docVIP

 PAGE \* MERGEFORMAT 20 Saturated fatty acids on pancreatic -cell apoptosis and cell cycle progression [Abstract] Objective To investigate the saturated fatty acids palmitic acid (palmitate acids, PA) on the MIN6 cell growth, proliferation and apoptosis, induction of apoptosis in MIN6 cells, inhibit cell cycle progression may be cellular and molecular mechanisms. Methods MTT method cell viability, cell cycle was determined by flow cytometry, Annexin / FITC / PI double staining flow cytometry to detect cell apoptosis. application of Western blot, cell cycle related protein cyclin D1 and CDK4 expression, and anti-apoptotic protein family (IAP) XIAP and cIAP 2 expression. Results and the control group: different concentrations of PA significantly inhibited MIN6 cell proliferation (P lt;0.01), the role of different concentrations of PA were in a time and dose dependent induction of MIN6 cells withered death (P lt;0.01), PA also significantly inhibited cell cycle progression, the more stuck in MIN6 G0/G1 cell cycle phase (P lt;0.01), G2 / M phase and S phase cells decreased (P lt;0.01), detected by Western blot: and the control group, PA decreased phosphorylation of AKT, XIAP, and cIAP 2 expression (P lt;0.01), and reduced cell cycle protein CDK4, cyclin D1 (P lt;0.01) expression levels. Conclusion The PA can inhibit the growth of islet cells, proliferation and induced apoptosis, possibly by reducing cyclin D1 and CDK4 activity, the cells remain in G0/G1 phase, inhibition of cell cycle progression and affect their reproduction, while reducing the anti-apoptotic protein the activity of XIAP and cIAP 2, to promote apoptosis. [Keywords:] proliferation and apoptosis in pancreatic islet cells unsaturated fatty acids ABSTRACT: Objective To investigate the effect of saturated fatty acid palmitate acids (PA) action on viability, proliferation and apoptosis of murine insulinoma MIN6 cells, and the possible intracellular pathways activated by PA. Methods Cell