Sassafras fat little rabbit model of alkali on lipid metabolism and fat PPAR INSIG2 gene expression.docVIP

 PAGE \* MERGEFORMAT 13 Sassafras fat little rabbit model of alkali on lipid metabolism and fat PPAR INSIG2 gene expression OF: He Qi Qi new Yang Junxia Alleviation Week [Abstract] Objective berberine on serum lipid metabolism in rabbits and its relationship with fat metabolism gene expression correlation. Methods fat high fat diet rabbit model established to determine serum levels of TC in each treatment , TG, LDL C and HDL C level. Real-time fluorescence quantitative PCR detection of adipose tissue gene expression of PPAR and INSIG 2. Results hyperlipidemia group TC, TG and LDL C levels were significantly higher than the normal diet (P lt;0.05, while the small Bo alkali treatment serum TC, TG and LDL C levels were significantly lower fat higher (P lt;0.05, real time quantitative PCR results showed that high-fat group and INSIG 2 mRNA expression of PPAR was significantly higher than the normal diet group (P lt; 0.05, berberine treatment was significantly higher expression of PPAR mRNA were significantly lower fat (P lt;0.05, while the INSIG 2 mRNA expression were significantly increased in high fat group (P lt;0.05, and the low dose effect is more obvious. Conclusion Berberine hypolipidemic effect has obvious, and its mechanism and the reduction in PPAR expression INSIG 2 gene upregulation. [Keywords:] berberine, high cholesterol, PPAR, INSIG 2 [Abstract] Objective To investigate the effects of berberine on lipids metabolism of hyperlipidemic rabbits, and to explore whether its mechanism was involved in the regulation of PPAR and INSIG 2 gene expressions in adipose tissue. Methods The rabbit hyperlipidemic model was established by feeding with high fat diet. The levels of serum total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL C) and high density lipoprotein cholesterol (HDL C) in each different treated group were determined. The mRNA expressions of PPARand INSIG 2 in adipose tissue were quantified by real time qu