Saturated flow-type solid-phase extraction method in the determination of paeoniflorin Xiaoyao pill.docVIP

 PAGE \* MERGEFORMAT 14 Saturated flow-type solid-phase extraction method in the determination of paeoniflorin Xiaoyao pill Author: Liaohong Juan, WANG Quan-Xi, Luo Yong, Wang Yi-fei 【Key Words】 Xiaoyao pill;,, paeoniflorin;,, solid phase extraction;,, high-performance liquid chromatography Abstract: Objective saturated flow-type solid-phase extraction (SPEHPLC) Preparation for the test items Xiaoyao pill solution, using high-performance liquid chromatography in which the content of paeoniflorin. Methods ZorbaxSBC18 column (4.6 mm × 250 mm, 5 μm), acetonitrile and water (20:80) as mobile phase, flow rate of 1 ml / min, detection wavelength was 230 nm. The results of the determination of paeoniflorin in the range of 0.123 2 ~ 1.232 μg good linear relationship, r = 0.999 9, the average recovery was 99.16%, RSD was 1.6%. Conclusion The method is simple, rapid, sensitive and effective to remove the protection of drug matrix column can be used to Xiaoyao pill in the determination of paeoniflorin. Keywords: Xiaoyao pill; paeoniflorin; solid-phase extraction; high performance liquid chromatography Content Determination of Peoniflorin in XiaoYao Pill by Saturated Flowthrough SPEHPLC Abstract: ObjectiveTo develop saturated flowthrough SPEHPLC method for the assay of Peoniflorin in XiaoYao pill. MethodsSeparation was performed on a ZorbaxSBC18 column (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of acetonitrile-water (20:80, v / v). The flow rate was 1 ml / min. The UV detection was set at 230 nm. ResultsPeoniflorin had a good linear relationship in the range of 0.123 2 ~ 1.232 μg, the average recovery of XiaoYao pill was 99.16% and the RSD was 1.6%. Conclusion The method is simple, sensitive and rapid , and reduce the damage of base material to the chromatographic column, it can be applied to the determination of peoniflorin in XiaoYao Pill. Keywords:: XiaoYao Pill; Peoniflorin; SPE; HPLC Xiaoyao pill (Shuiwan) is