SARS-CoV S240 protein and ACE2 receptor mechanism of the eye of.docVIP

 PAGE \* MERGEFORMAT 4 SARS-CoV S240 protein and ACE2 receptor mechanism of the eye of Authors: SUN Yan, Lin Liu, Xin Pan, Jing-ming [Abstract] Objective: To study the SARS coronavirus (SARS-CoV) S240 proteins and eye functional receptors - angiotensin-converting enzyme 2 (ACE2) role of the mechanism. Methods: Construction of SARS-CoV S protein fusion gene pS240-EGFP, and expression in mammalian cells; semi-quantitative RT-PCR and immunohistochemistry identification of ACE2 in the conjunctiva, cornea of the expression; enzyme-linked immunosorbent assay, flow cytometry method detected SARS-CoV S240 protein and conjunctiva, corneal cells in vitro combination studies. Results: To construct a fusion gene pS240-EGFP; confirmed that ACE2 in the conjunctiva, cornea to express; conjunctiva, corneal cells and SARS-CoV S240 protein can combine. Conclusion: The conjunctiva, cornea SARS coronavirus exists in the functional receptors, SARS-CoV S240 protein and conjunctiva, cornea cells to combine. In this study in understanding the pathological mechanism of SARS, and further study of the prevention and treatment of SARS has certain reference value. [Keywords:] severe acute respiratory syndrome coronavirus S240 protein receptor binding of angiotensin-converting enzyme 2 0 Introduction Atypical pneumonia or severe acute respiratory syndrome (severe acute respiratory syndrome, SARS), a highly infectious and pathogenic, its causative agent as a novel coronavirus-SARS coronavirus (SARS-CoV), genome structure as follows: 5’-cap-like structure - replicase (rep) - spike glycoprotein (S) - the small envelope protein (E) - membrane glycoprotein (M) - nucleocapsid protein (N) - polynucleotide (polyA) tail -3 ‘. S protein-mediated virus binding to host cells, SARS-CoV is a functional receptor angiotensin-converting enzyme 2 (angiotensin-converting enzyme 2-ACE2). In this study, expressed in 293T cells with SARS-CoV S protein-binding region of pS240-EGFP fusion protein fragment i