SELDI technical analysis of different liver cell line in vitro differences in protein expression.docVIP

 PAGE \* MERGEFORMAT 17 SELDI technical analysis of different liver cell line in vitro differences in protein expression Author: Ding Shou-yi, QIAN Dong-Meng, MU Wen-Feng, Yan Zhiyong, SONG Xu-Xia, Wang Bin [Key Words] surface-enhanced laser desorption / ionization / time of flight mass spectrometry; protein display analysis; liver cancer; tumor cells, cultured; differentially expressed proteins Analysis of different protein expressions in different liver cell strains in vitro using SELDI [Abstract] AIM: To examine the differential expressions of proteins in hepatoma cell line (HepG2), hepatoma cell line transfected by HBV (HepG2.2.15) compared with normal liver cell line (LO2) by using surfaceenhanced laser desorption / ionization timeofflight mass spectrometry (SELDITOFMS), so as to establish a foundation for further studying on the mechanism of hepatoma at the protein level. METHODS: The 3 types of cells above mentioned were cultured as general and collected when they were in good conditions. After cell disruption, SELDITOFMS was employed to detect the differential expressions of proteomes of HepG2, HepG 2.2.15 and LO2. RESULTS: Ninetyone proteins were captured by WCX2 array. Compared with normal liver cell lines, in the segments from Mr 5000 to 15 000, proteins in the HepG2 and HepG2.2.15 showed apparent changes, in which 2 proteins expressions were increased in carcinoma cells, the other 5 decreased. Nine proteins in HepG2 were increased and 10 proteins in HepG 2.2.15 were increased. CONCLUSION: The SELDI ProteinChip technology can be a desired method in detecting the biological markers in the earlier period of hepatoma. This method is of convenience, high sensitivity, and good reproducibility. The biological tags of tissuespecific proteins we found have a potential applying value in the early diagnosis of hepatoma. These tags are also meaningful in screening and identifying signal proteins from serum and tissue specimen in different types of hepatoma.