SELDI protein chip technology in the early diagnosis of cancer research.docVIP

 PAGE \* MERGEFORMAT 30 SELDI protein chip technology in the early diagnosis of cancer research Abstract SELDI protein chip technology, called the surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) was used to study proteomics in recent years, a new technology platform, with high-throughput, high efficiency, high-sensitivity and other characteristics, you can quickly analyze a variety of biological samples of protein composition of the group in basic medical research, clinical diagnosis and drug discovery and development aspects of the disease shows a wide application prospect and clinical significance. The article on its technology components, principles, characteristics, and in the early diagnosis of tumors and development prospects are reviewed. Keywords: Tumor Draft human genome sequence is completed, life science has entered a “post-genome era”, the focus turned to the study of complex protein function studies. Proteins are the end product of gene expression, the physiological change of state organs and tissues can cause changes in serum proteome, so protein and proteomics research can directly obtain the characteristic signs of disease. As the protein in human serum is a very rich and ever-changing system, a single serum tumor markers can not truly anti-dating back to the relevant cells and tissues, but found a group of cancer-related organizations, with a particular serum protein (multi-parameter marker) is a more effective way, which is the so-called “cancer fingerprint (cancer signature)” [1], and high-throughput surface-enhanced laser desorption ionization time of flight mass spectrometry (surface enhanced laser desorption / ionization time of flight mass spectrometry , SELDI-TOF-MS) mass spectrometry technologies has accelerated the conduct discovery “cancer fingerprint” studies. The use of SELDI technology is currently found in the specific tumor biological markers for early diagnosis