SELDI-TOF-MS technology in the early diagnosis of gastric cancer.docVIP

 PAGE \* MERGEFORMAT 13 SELDI-TOF-MS technology in the early diagnosis of gastric cancer [Key Words] surface-enhanced laser desorption ionization time of flight mass spectrometry; cancer Gastric cancer is the most common gastrointestinal cancer in China, accounting for the first cancer mortality [1]. Early detection, early diagnosis, early surgery is the primary means of improving the prognosis of gastric cancer. Current method of early diagnosis of gastric cancer in the number of surviving disadvantages, such as endoscopy will bring some pain, has been found associated markers in gastric cancer are carcinoembryonic antigen and carbohydrate antigen 19-9,125, with its sensitivity and specificity are low, are not sufficient to be used for early diagnosis [2]. surface-enhanced laser desorption ionization time of flight mass spectrometry (Surface-enhanced laser desorption ionization time-of-flight mass spectrometry, SELDI-TOF-MS) in early diagnosis of cancer, efficacy monitoring, prognosis and pathogenesis and so are showing broad application prospects [3]. SELDI-TOF-MS study of gastric cancer provides an ideal technology platform, now proteomics in cancer research are reviewed. 1 SELDI-TOF-MS technology principles Protein genes and cell function is the implementation of those carried out in recent years, proteomics research is an important post-genomic research era. In recent years the establishment of the SELDI-TOF-MS technology, also known as protein fingerprinting technology, with large-scale, ultra- trace, high-throughput, automated screening of protein and other characteristics, in order to provide an efficient proteomics research method. The technology is in early development of matrix-assisted laser desorption ionization time of flight mass spectrometry established on the basis of improved , including the protein chip, chip MS reader and analysis software, 3 [4-5]. protein chip according to the different surface materials can be divided into two