Shiga toxin-producing Escherichia coli producing extended spectrum β lactamase genotype.docVIP

 PAGE \* MERGEFORMAT 23 Shiga toxin-producing Escherichia coli producing extended spectrum β lactamase genotype 【Abstract】 Objective To investigate the Shiga toxin-producing Escherichia coli producing extended spectrum β  lactamases (extended spectrum β  lactamases, ESBLs) type, and resistance mechanisms. Methods filter paper agar diffusion method 36 Shiga toxin-producing E. coli strains producing ESBLs, conjugal transfer experiments, plasmid profile analysis, PCR detection of drug resistance genes and DNA sequence analysis of strains producing ESBLs phenotype and genotype. The results of double-disk diffusion method proved to be two Shiga toxin-producing Escherichia coli producing ESBLs, the U.S. strain of E.coli 5 nonO157: H7 and the Chinese strain of E.coli 27 O157: H7 to the ceftazidime and other third and fourth generation cephalosporin spore Abamectin and aminoglycoside antibiotic resistance, and can be passed to the receptor plasmid 5.8kb bacteria. PCR detection of genes containing blaTEM. DNA homology analysis showed that the 861bp of the blaTEM DNA fragment blaTEM  I DNA fragment homologous. Conclusion Shiga toxin-producing E. coli strains and China, the United States strains of the same type ESBLs, showed the third and fourth generation of β  lactam antibiotics resistance. Keywords: Shiga toxin-producing Escherichia coli extended-spectrum β  lactamase Study on the genotype of extended spectrum beta  lactamases among ABSTRACT Objective To investigate the genotype and antimicrobial mechanism of extended spectrum beta  lactamases among Shiga toxin  producing E.coli (STEC). Methods Antibiotic susceptibility was tested by the disk diffusion to screen out the strains that produced the ESBLs; conjugative transfer test, plasmid profile, PCR and DNA sequencing were used to detect the phenotype or genotype of STEC. Results Of 36 samples, there two isolates producing ESBL, E.coli 5 non O157: H7 from American and E.coli 27 O157