Study of vascular endothelial barrier function regulation.docVIP

 PAGE \* MERGEFORMAT 24 Study of vascular endothelial barrier function regulation Vascular endothelial barrier function of the adjustment mechanism is rather complicated. α -thrombin and other inflammatory medium increased endothelial permeability caused by the mechanism may be through G proteins to activate phospholipase, inositol triphosphate-mediated second messenger generation, and further activation of protein kinase C and myosin light chain kinase and eventually causing phosphorylation of myosin light chain, leading to endothelial cell F-actin cytoskeleton rearrangement, the center tension increased, cell fissure formation, endothelial cell permeability change. Abstract The regulatory mechanisms of vascular endothelial barrier function are complicated.Inflammatory mediators, such as-α -thrombin, activate phospholipases to mediate generation of IP3 ans other second messsengers through receptor-coupled G protein.Protein kinase C and myosin light chain kinase are then activated, leading to phosphorylation of myosin light chains, rearrangement of F-actin skeleton, interendothelial cell gap formation and increased endothelial permeability. Keywords: Barrier function; Endothelium; Permeablity; Regulation Including endothelial cells, vascular endothelial (EC) monolayer and the basement membrane is a vascular-cavity surface-layer of semi-selective permeability barrier. Blood vessels inside and outside the solute and fluid exchange of receptors control the size of endothelial permeability. Detection of endothelial permeability barrier function is an objective measurement of indicators. Number of inflammatory, as thrombotic (thrombogenic) media can damage endothelial barrier function, leading to increased permeability, plasma protein leakage, caused by the organization, organ edema and dysfunction. Vascular endothelial barrier dysfunction is one of the main features of inflammation and to participate in tumor metastasis, immune response and the occurrence of mu