Suicide gene system joint Liu Wei Di Huang Wan on the synergy of anti-hepatoma cells.docVIP

 PAGE \* MERGEFORMAT 22 Suicide gene system joint Liu Wei Di Huang Wan on the synergy of anti-hepatoma cells Author: Du S Yan ZHANG Ai-juan Yu-Hui Tan Yi-hua Wu Ying-Ya [Abstract] [Objective] Liu Wei Di Huang Wan-containing serum together HSV  tk / GCV suicide gene therapy system CBRH7919 anti-rat hepatoma cells, and explore the establishment of a suicide gene combined with traditional Chinese medicine prescriptions for cancer gene therapy, the feasibility of joint programs. [Methods] construct HSV  tk / GCV suicide gene therapy system to determine ganciclovir (GCV) working concentration (39.2μ mol / L); selected SD rats were fed prepared by Liu Wei Di Huang Wan-containing serum and blank serum, and to observe drug-containing serum and blank serum of tumor cells in vitro. Set up the blank control group, GCV control group, suicide gene therapy in the control group, these three groups were non-serum of the control group. The blank serum group, GCV plus blank serum group, suicide gene system plus blank serum group, drug-containing serum group, GCV plus drug-containing serum group, suicide gene system combined containing serum group, all rats of the blank serum and serum containing is further divided into volume fraction of 5% and 7.5% in two kinds of concentrations, each group re-established six holes. The rat hepatoma cells CBRH7919/tk and CBRH7919/tk- mixed, so that the proportion of tk cells accounted for 0%, 5%, 10%, according to 3 * 103 / hole cells were inoculated into 96-well plates, with full training Based cultured cells after 24h, corresponding to join the volume fraction of 5% or 7.5% of the rats with blank serum and the drug-containing serum, incubated 12h, adding GCV, cultivating 60h, tetrazolium salt (MTT) assay cell survival, with the Q value (measured the ratio of efficacy and theoretical efficacy) analysis of suicide gene system combined with the drug-containing serum whether the synergistic interaction of (0.85 ≤ Q amp;lt;1.15 for the addi