Summer without alkaloids pure high-speed countercurrent chromatography.docVIP

 PAGE \* MERGEFORMAT 16 Summer without alkaloids pure high-speed countercurrent chromatography Of: Wang Dai Jie Liu Jianhua Geng Yanling Wang Wen-Juan Fu Maorun paragraph [Abstract] pH zone refining countercurrent chromatography with conventional high-speed countercurrent chromatography method combining fast purification Xiatianwu total alkaloids. Using pH zone refining countercurrent chromatography for separation of total alkaloids from the summer without using hexane ethyl acetate methanol water (5:5:2:8, V / V, the sum of 5 mmol / L triethylamine, under the sum of 5 mmol / L HCl) as the solvent system, the sample size 3.0 g, isolated from a mixture and 3 high-purity single alkaloids: protopine (375 mg), Su Yuan Hu base A (362 mg) and dry than dry Spirit (246 mg), the purity of 97.5%, 95.6% and 97.1% . obtained by the conventional mixture of 850 mg the second separation speed countercurrent chromatography using hexane ethyl acetate methanol water (1:0.8:1.1:1, V / V) as solvent system, the spirit than is dry litter (105 mg) and four Hydrogen palmatine (470 mg) monomer, purity of 99.1% and 99.7%. from the Su-Yuan Hu medicine share a base, two separate models prepared by the combination of the summer to improve the efficiency of non-separation of alkaloids. [Keywords:] summer without; pH zone refining countercurrent chromatography; high-speed countercurrent chromatography; alkaloids Abstract A method was established for the separation and purification of alkaloids from the crude extract of Corydalis decumbens (Thunb.) Pers. Using high speed counter current chromatography (HSCCC) with two modes. First, pH zone refining counter current chromatography was successfully performed with a two phase solvent system, n hexane ethyl acetate methanol water (5:5:2:8, V / V), in which 5 mmol / L triethylamine in the organic stationary phase and 5 mmol / L hydrochloric acid in the aqueous mobile phase . From 3.0 g of crude extract, a mixture and three compounds, w