Traditional Chinese Medicine to adjust the process of precocious puberty in children study of the mechanism.docVIP

 PAGE \* MERGEFORMAT 31 Traditional Chinese Medicine to adjust the process of precocious puberty in children study of the mechanism Authors: CAI De-pei, Chen Boying, Zhang Wei, Li Bin [Keywords:], Chinese medicine [Abstract] Objective: Neuroendocrine regulation and gene expression point of view of adjustment of children in puberty precocious puberty process of filling Ziyin Xiehuo essence of Chinese medicine Chinese medicine and kidney effectively regulate hypothalamic-pituitary gonadotrophic function, the function of promoting the growth mechanism of . Methods: Adolescent rats were fed Ziyin Xiehuo be filled kidney essence of Chinese medicine or Chinese medicine. Use of experimental methods in neurobiology (hypothalamic push-pull perfusion, tissue, brain slices were incubated and immunohistochemical staining) to detect hypothalamic gonadotropin-releasing hormone gonadotrophic zone (gonadotropinreleasing hormone, GnRH) levels, pulse frequency and the release of amplitude, as well as the central amino acid neurotransmitters, neuropeptide Y and β -endorphin release changes; using quantitative reverse transcription-polymerase chain reaction detection of hypothalamic GnRH, growth hormone releasing hormone (growth hormone releasing hormone, GHRH), somatostatin ( somatostatin, SS), anterior pituitary gland follicle-stimulating hormone (follicular stimulating hormone, FSH), luteinizing hormone (luteinizing hormone, LH), growth hormone (growth hormone, GH), long bone metaphysis insulin-like growth factor-Ⅰ (insulinlike growth factor Ⅰ , IGF Ⅰ ) gene and protein expression levels. Results: Ziyin Xiehuo medicine can inhibit the hypothalamic gonadotropic areas of the release of excitatory amino acid neurotransmitters to promote the inhibitory amino acid neurotransmitter, neuropeptide Y and β -endorphin release, so that hypothalamic GnRH neurons in functional activities reduced, reduced GnRH, FSH and LH gene expression level, increase hypothalamic SS gene expression,