The long-term implantation of depleted uranium in rats Effect of renal injury in.docVIP

 PAGE \* MERGEFORMAT 21 The long-term implantation of depleted uranium in rats Effect of renal injury in 【Abstract】 Objective To study the depleted uranium at different time points after implantation of the rat kidney damage effects. Methods in early adult healthy male Wistar rats were implanted depleted uranium fragments were observed in rats after exposure to depleted uranium on the kidney 3,6,12,18 ultrastructure and morphological changes in renal function tests and collagen staining, and use immunofluorescence and protein blot hybridization detection of renal fibrosis in different time related factor TGF-β 1 and Smad expression. The results of implantation of depleted uranium in rats at different time periods there are different degrees of renal pathological changes in glomeruli showed pyknosis, tubular atrophy and partial follow-up of renal interstitial fibrosis and inflammatory cell infiltration. Fibrosis is aggravated with prolonged exposure. Collagen staining showed the distribution of collagen are mainly located around the tubular, and has regional. TGF-β 1 and Smad is the process of renal fibrosis important cytokines. Western Blot analysis shows that depleted uranium exposure in rat kidney TGF-β 1 and Smad have varying degrees of growth. Conclusion depleted uranium long after implantation can occur in rat renal pathology and ultrastructural changes, TGF-β 1 and Smad participate in its fibrosis. Keywords: depleted uranium toxicity renal fibrosis Abstract: Objective To study the toxic effects on rat kidney after long term depleted uranium (DU) exposure.Methods Twenty adult male rats were implanted DU plates as implanted group.The morphology changes of kidneys were observed by electronmicroscopy and microscopy after 3,6, 12,18 months exposure.Fibrosis-correlated cytokines such as TGF-β1 and Smad were analyzed by immunofluorescence and Western Blotting.Kidney function and collagen staining were also fulfilled.Results At different time