Water-soluble CdSe - CdS core-shell nanocrystals with Bovine Serum Albumin.docVIP

 PAGE \* MERGEFORMAT 13 Water-soluble CdSe / CdS core-shell nanocrystals with Bovine Serum Albumin Author: Lin-fang, Huang Feng-Hua, Lan Ya-ling, Cheng Chen, Rui Wan [Abstract] Objective To explore the cysteine (Cys) of Surface-Modified CdSe / CdS core-shell nanocrystals (CdSe / CdS / Cys) in the tag in the application of bovine serum albumin. Methods UV and fluorescence spectroscopy CdSe / CdS / Cys nanocrystals with bovine serum albumin interactions. The results mark bovine serum albumin CdSe / CdS / Cys nanocrystals fluorescence emission peak intensity increased markedly. Conclusion This new CdSe / CdS / Cys nanocrystals can be used to mark bovine serum albumin, bovine serum albumin marking the CdSe / CdS / Cys changes in the absorption and emission spectra may be due to coordination between the two, as well as electrostatic interactions. [Keywords:] nanostructure; biological markers; bovine serum albumin; Spectroscopy Because the semiconductor nanocrystals with a quantum size effect and surface effect, and thus demonstrated the unique physical and chemical properties, with excellent spectral characteristics and photochemical stability, as a fluorescent biological probes, the potential application of bio-medical aspects of the value of scientific work caused by are of great concern [1  5]. In 1998, Alivisators such as the first report of the semiconductor nanocrystals as a fluorescent probe of mouse tissue tagging [1], nano-crystalline in the biological sciences applications quickly aroused interest. However, as the early nanocrystals is obtained in the organic phase and therefore do not dissolve in water, not suitable for biological applications, this approach must be obtained through the modification of nano-crystalline transferred to the aqueous phase, while the modification often makes light-emitting nano-crystalline quantum yield decreased [4]. Direct synthesis in aqueous solution with a high fluorescence quantum yield, light and stable water-so