乳腺癌组织中PCNA、bcl.docVIP

　　乳腺癌组织中PCNA、bcl 作者：宾晓农　谭敏　何青莲　赵虹　刘鹏熙 【摘要】 　　［目的］ 探讨乳腺癌、癌旁和正常乳腺组织中PA、bcl-2与p53基因蛋白表达的差异及其在乳腺癌变中的意义。［ 方法 ］ 用免疫组化法检测46例乳腺癌、癌旁组织和33例正常乳腺组织中PA、bcl-2与p53基因蛋白表达。［结果］ ①PA指数：乳腺癌与癌旁组织差异无显著性；乳腺癌和正常乳腺组织(46.03±8.76与32.13±6.78)，乳腺癌旁和正常乳腺组织(42.90±7.92与32.13±6.78)差异均有显著性(Plt;0.01)。②bcl-2基因蛋白表达分值：乳腺癌、癌旁和正常乳腺组织分别为：0.62±0.47，0.91±0.58，1.56±0.84；分值大小顺序呈表达上升趋势。③p53基因蛋白表达分值：乳腺癌、癌旁组织和正常乳腺组织分别为：2.21±0.96，1.86±0.87，0.87±0.57；分值大小顺序表达呈下降趋势。④在乳腺癌组织PA指数与bcl-2呈中度负相关(rs=-0.537)，与p53呈中度正相关(rs=0.628)，bcl-2与p53呈中度负相关(rs=-0.476)。［结论］ 乳腺癌的发生、 发展 过程中PA和p53分值增大，bcl-2分值变小，预示组织恶性程度可能增加，对判定乳腺组织的良、恶性及预后具有重要临床价值。 【关键词】 乳腺肿瘤　增殖细胞核抗原　bcl-2　p53 　　 Expression of PA, bcl-2 and p53 ProtEins in Breast Cancer Abstract: ［Purpose］ To explore difference of expression of PA, bcl-2 and p53 protEIns among breast carcinoma, para-carcinoma tissue and normal breast tissue and its value in development of breast carcinoma. ［Method］ PA, bcl-2 and p53 proteins in carcinoma tissue and para-carcinoma tissue in 46 patients ples of normal breast tissue munohistochemical S-P method. ［Results］ ①PA-PI shoa and para-carcinoma tissue (Pgt;0.05), but significant differences a and the normal breast tissue (46.03±8.76 vs. 32.13±6.78), and beta tissue and the normal breast tissue (42.90±7.92 vs. 32.13±6.78, Plt;0.01). ② The expression score of bcl-2 a, 0.91±0.58 in para-carcinoma tissue and 1.56±0.84 in normal breast tissue, cancer to normal tissue. ③The expression score of p53 a tissue, para-carcinoma tissue and the normal breast tissue, cancer to normal tissue. ④PA-PI shooderately negative correlation oderately positive correlation oderately negative correlation a tissue. ［Conclusion］ Increases of PA and p53, decrease of bcl-2 predict the tissue malignant potential during occurrence and development of breast carcinoma, portant role for diagnosis and prognosis. Key s; proliferative cell nuclear antigen; bcl-2; p53 关于乳腺癌发生、发展的确切机制尚未完全清楚，因而解决其诊治及预后评估等 问题 需要通过系统的 分析 ， 研究 肿瘤组织内在的复杂 规律 ，以便更快地找到有助于指导乳腺癌诊治的可靠方法，并采用综合分析手段，从单基因、单指标走向多基因指