硒化壳聚糖通过下调PML.docVIP

　　硒化壳聚糖通过下调PML 作者：邓守恒，曹凤军，蔡晓军，许涛，邓守平，陈萍 【摘要】 目的研究硒化壳聚糖对急性早幼粒白血病细胞株NB4增殖和凋亡的影响，探讨这种影响与PML-RARα融合蛋白表达的关系。方法应用MTT法检测硒化壳聚糖对细胞增殖的影响，应用AO/EB荧光染色法共聚焦显微镜下观察诱导凋亡作用，应用L-RARα融合蛋白含量的变化。结果硒化壳聚糖可对体外培养的NB4细胞产生剂量和时间依赖性的抑制作用，50 mg/L 至 100 mg/L硒化壳聚糖作用NB4细胞24 h可诱导细胞出现明显的凋亡现象，经硒化壳聚糖处理24 h后，NB4细胞内PML-RARα融合蛋白含量明显减少。结论硒化壳聚糖可通过下调PML-RARα融合蛋白含量对NB4细胞产生抑制增殖和诱导凋亡作用。 【关键词】 硒化壳聚糖; NB4细胞；增殖 ；凋亡； PML-RARα融合蛋白 　Chitosan ［β(1,4)-2-amino-2-deoxy-D-glucose］, an almost nontoxic bio-polysaccharide in nature, acological effects including antitumor, antimicrobial and antioxidant properties［1,2］ by report, is often used as drug preparation materials due to its excellent biopatibility［3］. Selenium, belonging to the same family in the periodic table of elements or element (Arsenic trioxide has long been used as an anti-leukemia drug in clinical practice)has been found to increase apoptosis of Acute Promyelocytic Leukemia (APL) cell lines NB4 cell through PI3K/Akt path can not be easily absorbed by cancer cells , have narroall lethal dose, and so on. Therefore, , i. e. Chitosan selenium ［5］, via chitosan and selenious acid in acid solution, using the mixed metal ions as the catalyst, , should also have anti-leukemia effect and have much smaller side effects than inorganic selenium and arsenide［6］. 　　In this chitosan on proliferation and apoptosis of human APL cell lines NB4 cell in vitro．And the ability of Selenium chitosan to modulate PML-RARα fusion protEins that might contribute to NB4 cell survival ethods 　　1.1 Drugs and antibodiesSelenium chitosan ent of pharmaceutical Chemistry， Fujian medical University；Selenium content Santa Cruz Biotechnology．I-l640 medium supplemented ，streptomycin100 mg/L，benzylpenicillin 100 kU/Lat 37% in a humidified 5%CO2．After incubation for 24 h, exponentially gro chitosan of different concentrations (50,l00,200 mg/L) for 24 h～48 h．MTT ine the proliferative effects of drugs on tumor cells and AO/EB fluoresent staining method Selenium chitosan-treated cells mol/L