祛湿化瘀方对脂肪肝大鼠脂联素游离脂肪酸代谢路径的影响.docVIP

　　祛湿化瘀方对脂肪肝大鼠脂联素游离脂肪酸代谢路径的影响 作者：李红山, 冯琴, 许丽莉 陈少东 李雪梅, 胡义扬 【摘要】 目的：探讨祛湿化瘀方防治脂肪肝的作用及其对脂联素（adiponectin, ADP）游离脂肪酸（free fatty acid, FFA）代谢路径的影响。方法：采用Pactivated protEin kinase, AMPK）、脂肪酸合成酶（fatty acid synthase, FAS）、乙酰辅酶A羧化酶（acetylcoenzyme A carboxylase, ACCase）、肉毒碱脂酰转移酶1（carnitine palmitoyltransferase1, CPT1）含量的变化。 结果：与正常组比较，模型组大鼠肝组织出现明显大泡样脂肪变性；肝组织TG、FFA、FAS、ACCase和malonylCoA含量显著升高，血清ADP及肝组织AdipoR2、AMPK和CPT1含量明显降低。高、中、低剂量祛湿化瘀方均可使脂肪肝大鼠肝脏的病理改变显著减轻，肝组织TG、FFA含量降低；高剂量祛湿化瘀方可使模型大鼠肝组织FAS、ACCase和malony1CoA含量显著降低；高、中剂量祛湿化瘀方均能明显升高血清ADP和肝组织AdipoR2、AMPK、CPT1含量。结论：祛湿化瘀方可通过提高血清脂联素水平，改善低脂联素血症，影响ADPFFA代谢路径 治疗 大鼠脂肪肝。 【关键词】 脂肪肝; 游离脂肪酸; 脂联素; 腺苷酸活化蛋白激酶; 祛湿化瘀方; 大鼠 Methods: Forty Pactivated protein kinase (AMPK), acetylCoA carboxylase (ACCase), fatty acid synthase (FAS) and carnitine palmitoyl transferase1 (CPT1). Results: pared al group, there mation in liver tissues in the untreated group as observed by oil red O staining and HE staining. The TG, FFA, malony1CoA, FAS, and ACCase concentrations in liver tissues in the untreated group and AdipoR2, CPT1 and AMPK in liver tissues in the untreated group arkedly. The pathological damages in each QSHYDtreated group alonylCoA concentrations in liver tissues of the high QSHYDtreated group arkedly as pared ediumdose of QSHYD could significantly increase ADP content in serum and AMPK, CPT1 and AdipoR2 contents in liver tissues. Conclusion: QSHYD can affect the ADPFFA path ADP, ay be one of its important mechanisms in preventing and treating NAFLD in rats. KeyL的药液，冷藏备用。分析纯CCl4和化学纯橄榄油，均购自中国医药集团上海化学试剂公司；血清丙氨酸氨基转移酶（alanine aminotransferase, ALT）、天冬氨酸氨基转移酶（aspartate aminotransferase, AST）和FFA检测试剂盒，均购自南京建成生物工程研究所；甘油三酯（triglyceride, TG）检测试剂盒，购自浙江东欧生物工程有限公司；大鼠血清ADP，肝组织脂联素受体2（adiponectin receptor 2, AdipoR2）、腺苷酸活化的蛋白激酶（AMPactivated protein kinase, AMPK）、脂肪酸合成酶（fatty acid synthase, FAS）、乙酰辅酶A羧化酶（acetylCoA carboxylase, ACCase）、丙二酰辅酶A（malonylcoenzyme A, malonylCoA）、肉毒碱棕榈酰基转移酶1（carnitine palmitoyltransferase1, CPT1）酶联免疫吸附