Conjugation of the Recombinant Third Domain of Human Alpha-fetoprotein with Doxorubicin Using Poly(amidoamine) Dendrimers and Study of Its Cytotoxic Activity.docVIP

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Conjugation of the Recombinant Third Domain of Human Alpha-fetoprotein with Doxorubicin Using Poly(amidoamine) Dendrimers and Study of Its Cytotoxic Activity.doc

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Conjugation of the Recombinant Third Domain of Human Alpha-fetoprotein with Doxorubicin Using Poly(amidoamine) Dendrimers and Study of Its Cytotoxic Activity

Engineering, 2012, 5, 80-83 doi:10.4236/eng.2012.410B021 Published Online October 2012 (http://www.SciRP.org/journal/eng) Conjugation of the Recombinant Third Domain of Human Alpha-fetoprotein with Doxorubicin Using Poly(amidoamine) Dendrimers and Study of Its Cytotoxic Activity S. I. Kisil , V. A. Chernikov , M. I. Danilevskiy , L. V. Savvateeva , N. V. Gorokhovets 1 2 2 3 4 1 Biochemistry department, I.M. Sechenov First Moscow State Medical University, Moscow, Russia Research Institute of Uronephrology and Reproductive Health Care of the I. M. Sechenov First MSMUk, Moscow, Russia National Research Centre “Kurchatov Institute”, Moscow, Russia Research Institute of Molecular Medicine of the I. M. Sechenov First MSMU, Moscow, Russia Email: ksi-5826@mail.ru 2 3 4 Received 2012 ABSTRACT The recombinant third domain of alpha-fetoprotein (AFP3D) is one of the promising vectors for targeted delivery of anticancer drugs to tumor cells. AFP3D-based conjugates with various anti-tumor agents have shown specific anti-tumor activity. The content of an- ti-tumor drugs in the preparation could be increased by including dendrimers - highly branched nanoparticles - into a targeted deli- very system. In this study, we synthesized the AFP3D conjugate with doxorubicin-loaded poly(amidoamine) (PAMAM) dendrimer nanoparticles and investigated its cytotoxic activity. Keywords: Alpha-fetoprotein; Dendrimer; Doxorubicin; Targeted Delivery 1. Introduction Ferein (Russia). Dendrimer PAMAM G-3.5 was purchased from Sigma-Aldrich (USA). Cancer targeted therapy represents one of the most rapidly de- veloping areas in pre-clinical and clinical cancer research. The recombinant third domain of alpha-fetoprotein (AFP3D) is one of the promising vectors for targeted delivery of