Core Oligosaccharide of Plesiomonas shigelloides PCM 2231 (Serotype O17) Lipopolysaccharide — Structural and Serological Analysis.docVIP

Mar. Drugs 2013, 11, 440-454; doi:10.3390/mOPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article Core Oligosaccharide of Plesiomonas shigelloides PCM 2231 (Serotype O17) Lipopolysaccharide—Structural and Serological Analysis Anna Maciejewska *, Jolanta Lukasiewicz , Marta Kaszowska , Aleksandra Man-Kupisinska 1, 1, 1 1 1 Wojciech Jachymek and Czeslaw Lugowski 1,2 1 Department of Immunochemistry, Ludwik Hirszfeld Institute Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wroclaw, Poland; E-Mails: czaja@iitd.pan.wroc.pl (J.L.); marta.kaszowska@iitd.pan.wroc.pl (M.K.); aleksandra.man@iitd.pan.wroc.pl (A.M.-K.); jachymek@iitd.pan.wroc.pl (W.J.); lugowski@iitd.pan.wroc.pl (C.L.) 2 Department of Biotechnology and Molecular Biology, University of Opole, Kardynala Kominka 6a, 45-032 Opole, Poland * Author to whom correspondence should be addressed; E-Mail: aniaaugustyniuk@iitd.pan.wroc.pl; Tel.: +48-71-370-9927; Fax: +48-71-337-1382. Received: 17 December 2012; in revised form: 8 January 2013 / Accepted: 22 January 2013 / Published: 6 February 2013 Abstract: The herein presented complete structure of the core oligosaccharide of lipopolysaccharide (LPS) P. shigelloides Polish Collection of Microorganisms (PCM) 1 13 2231 (serotype O17) was investigated by H, C NMR spectroscopy, mass spectrometry, chemical analyses and serological methods. The core oligosaccharide is composed of an undecasaccharide, which represents the second core type identified for P. shigelloides serotype O17 LPS. This structure is similar to that of the core oligosaccharide of P. shigelloides strains 302-73 (serotype O1) and 7-63 (serotype O17) and differs from these only by one sugar residue. Serological screening of 55 strains of P. shigelloides with the use of serum against identified core oligosaccharide conjugated with bovine serum albu