Culture condition, inoculum production and host response of a wild mushroom, Phlebopus portentosus strain CMUHH121-005.docVIP

Maejo Int. J. Sci. Technol. 2010, 5(03), 413-425 Maejo International Journal of Science and Technology ISSN 1905-7873 Available online at www.mijst.mju.ac.th Full Paper Culture condition, inoculum production and host response of a wild mushroom, Phlebopus portentosus strain CMUHH121-005 Naritsada Thongklang , Kevin D. Hyde , Boonsom Bussaban and Saisamorn Lumyong 1 2, 3 1 1,* 1 2 3 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand School of Science Mae Fah Luang University, Chiang Rai 57100, Thailand Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia * Corresponding author, e-mail: scboi009@chiangmai.ac.th Received: 7 July 2010 / Accepted: 15 November 2011 / Published: 9 December 2011 Abstract: The optimal media and growth conditions of an edible wild mushroom, Phlebopus portentosus strain CMUHH121-005, were investigated for inoculum production. The optimum temperature for mycelial growth was 30oC and the optimum pH was 4.0. Malt extract and yeast extract as carbon and nitrogen sources respectively, with a C:N ratio of 10:1, were most suitable. The biomass production in 16 types of cereal grain media was investigated. The fungus was found to grow best in barley grain mixed with Murashige and Skoog solution over 30 days following inoculation. Further incubation at 30°C for 60 days in the dark caused numerous agglomerations of mycelia as if fruiting bodies were being formed. The data presented provide growth requirements that will be useful in a future development of P. portentosus as a cultivatable mycorrhizal mushroom. Keywords: Phlebopus portentosus, mushroom cultivation, mycorrhizae, mushroom inoculum