Effect of a novel compound from.docVIP

Vol.5, No.1, 84-92 (2013) Natural Science /10.4236/ns.2013.51014 Effect of a novel compound from Lycopodium obscurum L. on osteogenic activity of osteoblasts in vitro Chaoyuan Wang , Ruiyun Wu , Guangzhong Yang , Keith A. Blackwood , Thor Friis , 1,2 1,2 3 4 4 4 4* Dietmar W. Hutmacher , Maria Ann Woodruff 1 Engineering Research Centre for the Protection and Utilization of Bioresource in Ethnic Area of Southern China, South-Central University for Nationalities, Wuhan, China 2 3 4 School of Life Science, South-Central University for Nationalities, Wuhan, China School of Pharmacology, South-Central University for Nationalities, Wuhan, China * IHBI, Queensland University of Technology, Brisbane, Australia; Corresponding Author: mia.woodruff@.au Received 30 October 2012; revised 28 November 2012; accepted 9 December 2012 ABSTRACT and traditional Tu medicine to treat arthritis pain, quad- riplegia, dysmenorrheal and contusion [1]. It is also used in treatment of bone fractures [2,3]. However, little is known about the pharmacology of Lycopodium obscu- rum L. in bone fracture healing, which prevents its ap- plication in western medical practices. We investigated the potential of an extract of Lycopodium obscurum L.; stigmastane-3-oxo- 21-oic acid (SA), to enhance osteogensis of mouse osteoblastic MC3T3-E1 cells. SA at a concentration of 16 μM was found to have no significant effect upon the viability of the cells, thus concentrations of 8 μM and 16 μM of SA were used in all further experiments. Both con- centrations of SA had an inhibitory affect upon alkaline phosphatase activity (ALP) after 8 days incubation, however, after 16 days activity was restored to control