Effect of different temperature conditions on survival time of Linguatula serrata nymphs.docVIP

HVM BIOFLUX Human Veterinary Medicine International Journal of the Bioflux Society Effect of different temperature conditions on survival time of Linguatula serrata nymphs 1 1 2 Afshin Akhondzadeh Basti, Hamidreza Haddadzadeh, Hossein Tajik, 2 3 2 4 Bahador Hajimohammadi, Saloomeh Shirali, Mohammad Hemati, and Emad Ahmadiara 1 Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of 2 Tehran, Tehran, Iran; Department of Parasitology, Faculty of Veterinary Medicine, 3 University of Tehran, Tehran, Iran; Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran; Faculty of Veterinary 4 Medicine, University of Tehran, Tehran, Iran. Corresponding author: B. Hajimohammadi, hajimohammadib@ut.ac.ir Abstract. Objective: Linguatulosis is one of the food-borne parasitic zoonoses. Human infection may occurs following consumption of the raw or undercooked infected offal of slaughtered ruminants, especially mesenteric lymph nodes (MLNs), liver and lung. The main objective of this study was to evaluate the effect of different temperature conditions on survival time of Linguatula serrata nymphs. Material and Methods: Fifteen infected MLNs (5 in triplicate) were separately put in plastic bags and were treated by +50°C, +60°C, +72°C and -18°C through water bath or freezer. Meanwhile, fifteen infected MLNs (5 in triplicate) were stored in refrigerator (+4°C) as control group. The survival time of the nymphs was evaluated by observing their motility and wriggle under a stereomicroscope in different periods of time. Results: The infected MLNs were mainly edematous, hemorrhagic and dark comparing to the non-infected ones. The survival time of the nymphs stored at the high temperat