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Effect of Dual Inoculation of Arbuscular Mycorrhiza and
Advances in Microbiology, 2012, 2, 561-564
/10.4236/aim.2012.24072 Published Online December 2012 (http://www.SciRP.org/journal/aim)
Effect of Dual Inoculation of Arbuscular Mycorrhiza and
Rhizobium on the Chlorophyll, Nitrogen and Phosphorus
Contents of Pigeon Pea (Cajanus cajan L.)
Sujata Bhattacharjee , Gouri Dutta Sharma
*
Department of Life Science and Bioinformatics, Assam University, Silchar, India
Email: bhattacharjee.sujata819@
*
Received August 27, 2012; revised October 8, 2012; accepted October 17, 2012
ABSTRACT
The pot experiments were conducted in the Department of Life Science and Bioinformatics of Assam University, Sil-
char. The aim of the present study was to investigate the effect of dual inoculation of Arbuscular mycorrhiza (AM)
(Glomus fasciculatum) and Rhizobium on the chlorophyll, nitrogen and phosphorus contents of pigeon pea (Cajanus
cajan L.). The results revealed an overall increase in chlorophyll, nitrogen and phosphorus contents in the inoculated
plants than uninoculated ones. Maximum chlorophyll contents were recorded in the plants dually inoculated with
Glomus fasciculatum and Rhizobium. The combined application of Glomus fasciculatum and Rhizobium also remarka-
bly increased the nitrogen and phosphorus contents of pigeon pea. The dual inoculation with microsymbionts revealed
synergistic effect. The results suggest that dual inoculation of Glomus fasciculatum and Rhizobium have the potential to
enhance the chlorophyll, nitrogen and phosphorus contents of pigeon pea.
Keywords: Glomus fasciculatum; Rhizobium; Dual Inoculation; Chlorophyll; Nitrogen; Phosphorus
1. Introduction
tion in Chickpea due to dual inoculation with G. fascicu-
latum and Rhizobium was reported by Subba Rao et al.,
1985 [8]. In the light of these views, an attempt has been
made in the present study, to investigate the effect of
dual inoculation of AM fungus (Glomus fasciculatum)
and Rhizobium in c
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