活性黑5脱色菌的分离鉴定及其脱色特性_陈刚.pdf

DOI :10.159 5/j .cnki.1001 -3 65.2010.12.013 　　5 活性黑5 脱色菌的分离鉴定及其脱色特性＊ 陈　刚　陈　晔　黄满红　陈　亮 (, 201620) 2 　　　A O 1 5 GY-1, 、 16S rDNA , (E nterobacter)。5 , 5 :8%()、pH 7.0、35 ℃。, GY-15 , 5 25 ～ 100 mg/L 。 　　　5 　　　16S rDNA Isolation, identification and decolorization characteristics of Reactive Black 5 decolorizing strain　CH EN Gang, CHE N Ye, H UAN G Manhong, CHE N L iang.(College of Environmental S cience and Engineering, Donghua University, Shanghai 201620) Abstract:　Reacti e Black 5 decolorizing strain GY-1 w as isolated from the anaerobic sludge which applied in 2 modified anaerobic-anox ic-aerobic (A O)process for dyeing and finishing w astewater treatment.According to the morphological characteristics, physiological and biochemical, and sequence analy sis of 16S rDNA, the strain w as identified preliminarily as Enterobacter.The decolorizing of Reacti e Black 5 by GY-1 w as in estigated to determine the effect of inoculated quantity, pH and temperature on decolorization efficiency .The optimum decolorization condi- tions were obtained as follow:inoculum amount was 8%, pH was 7.0, and 35 ℃.When in the initial dye concentra- tions ranged from 25 to 100 mg/L, the decolorization reaction can be described by one order kinetic characteristic. Keywords:　Reacti e Black 5;decolorization;decolorizing strain identification;16S rDNA [6] 　　、 、, 。 ,,、、 。, 80 ～90 , 、 [1] [7, 8] 2 t, 15 t, 。 。A O 10%(, )～ 15 GY-1, [2] 20% 。 、、16S rDNA 、、、 , 5 , , 5 。 [3] 。 1　 ,。 , 、 1.1　主要材料