Analysis of Transcripts Expressed in One-Day-Old Larvae and Fifth Instar Silk Glands of Tasar Silkworm, Antheraea mylitta英文文献资料.docVIP

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Analysis of Transcripts Expressed in One-Day-Old Larvae and Fifth Instar Silk Glands of Tasar Silkworm, Antheraea mylitta英文文献资料.doc

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Analysis of Transcripts Expressed in One-Day-Old Larvae and Fifth Instar Silk Glands of Tasar Silkworm, Antheraea mylitta英文文献资料

HindawiPublishingCorporation ComparativeandFunctionalGenomics Volume2010,ArticleID246738,11pages doi:10.1155/2010/246738 ResearchArticle AnalysisofTranscriptsExpressedinOne-Day-OldLarvaeand FifthInstarSilkGlandsofTasarSilkworm,Antheraeamylitta SamitaMaity, 1 SagarI.Goel, SobhanRoy, SuvankarGhorai, SwatiBhattacharyya, 1 1 1 1 andAnantaK.Ghosh 1 AravindVenugopalan, 2 1 2 DepartmentofBiotechnology,IndianInstituteofTechnology,Kharagpur721302,India DepartmentofElectricalEngineering,IndianInstituteofTechnology,Kharagpur721302,India CorrespondenceshouldbeaddressedtoAnantaK.Ghosh,aghosh@hijli.iitkgp.ernet.in Received3September2009;Accepted3February2010 AcademicEditor:JohnParkinson Copyright?2010SamitaMaityetal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited. Antheraeamylittaisoneofthewildnonmulberrysilkworms,whichproducestasarsilk.AnESTprojecthasbeenundertakento understandthegeneexpressionpro?leofA.mylittasilkgland.TwocDNAlibraries,onefromthewholebodiesofone-day-old larvaeandtheotherfromthesilkglandsof?fthinstarlarvae,wereconstructedandsequenced.Atotalof2476good-qualityESTs (1239clones)wereobtainedandgroupedinto648clusterscontaining390contigsand258singletonstorepresent467potential unigenes.Forty-?vesequencescontainedputativecodingregion,andrepresentedpotentiallynovelgenes.Amongthe648clusters, 241werecategorizedaccordingtoGeneOntologyhierarchyandshowedpresenceofseveralsilkandimmune-relatedgenes.TheA. mylittaESTshavebeenorganizedintoafreelyavailableonlinedatabase“AmyBASE”.Thesedataprovideaninitialinsightintothe A.mylittatranscriptomeandhelptounderstandthemolecularmechanismofsilkproteinproductioninaLepidopteranspecies. 1.Introduction is declining due to reasons like deforestation, pathogenic infection and socio-economic problems. The cytoplasmic polyhedrosis virus infection alone leads