Analytical Expressions Pertaining to the Concentration of Substrates and Product in Phenol-Polyphenol Oxidase System Immobilized in Laponite Hydrogels A Reciprocal Competitive Inhibition Process英文文献资料.docVIP

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Analytical Expressions Pertaining to the Concentration of Substrates and Product in Phenol-Polyphenol Oxidase System Immobilized in Laponite Hydrogels A Reciprocal Competitive Inhibition Process英文文献资料.doc

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Analytical Expressions Pertaining to the Concentration of Substrates and Product in Phenol-Polyphenol Oxidase System Immobilized in Laponite Hydrogels A Reciprocal Competitive Inhibition Process英文文献资料

HindawiPublishingCorporation AdvancesinPhysicalChemistry Volume2012,ArticleID212818,11pages doi:10.1155/2012/212818 ResearchArticle AnalyticalExpressionsPertainingtotheConcentrationof SubstratesandProductinPhenol-PolyphenolOxidaseSystem ImmobilizedinLaponiteHydrogels:AReciprocalCompetitive InhibitionProcess K.IndiraandL.Rajendran DepartmentofMathematics,TheMaduraCollege,Madurai625011,India CorrespondenceshouldbeaddressedtoL.Rajendran,raj sms@redi? Received6October2011;Accepted5December2011 AcademicEditor:MarcKoper Copyright?2012K.IndiraandL.Rajendran.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. Theoretical analysis corresponding to the di?usion and kinetics of substrate and product in an amperometric biosensor is developedandreportedinthispaper.Thenonlinearcoupledsystemofdi?usionequationswasanalyticallysolvedbyHomotopy perturbationmethod.Herein,wereporttheapproximateanalyticalexpressionspertainingtosubstrateconcentration,product concentration,andcurrentresponseforallpossiblevaluesofdi?usionandkineticparameters.Thenumericalsolutionofthis problemisalsoreportedusingScilab/Matlabprogram.Also,wefoundexcellentagreementbetweentheanalyticalresultsand numericalresultsuponcomparison. 1.Introduction Blaedel et al. [4] derived equations for steady-state ?uxes of substrates and product through a membrane in simple systems. Theoretical modeling of biosensors usually provides some important insight into understanding the functioning of a biosensor. Usually, with the aid of an analytical device, it is not possible to measure the concentration of substrates insidetheenzymemembranes.Asaresult,theoreticalmodel inbiosensorshasbeendevelopedandemployedasanimpor- tanttooltostudytheanalyticalcharacteristicsofbiosensors. Initially, Goldman et al. [1] reported the theoretical mod- elingonbiosensor.Inthatreport[1],theyhavepublished anextensivetheor