Application of on-line quality control for salvianolic acid B by near infrared spectroscopy英文文献资料.docVIP

Vol.1, No.3, 244-248 (2009) Health doi:10.4236/health.2009.13040 Application of on-line quality control for salvianolic acid B by near infrared spectroscopy Jin-Wei Zhang*, Yan Liu, Wei-Wei Liu, Yan-Ying Zhang Tasly Modern TCM Resources Co., LTD., Tianjin, China; tjvv@163.com Received 11 August 2009; revised 23 September 2009; accepted 22 October 2009 ABSTRACT As the chemistry metrology and computer technology develop, NIR has already been widely adopted in agri- culture and petroleum industries [1-2]. In 2002, USFDA approved NIR to be one of the standard measurement methods. In 2005, “China Pharmacopoeia” listed “NIR Spectroscopy instruction” in [3]. NIR Spectroscopy technology turns out to be a research hotspot in on-line testing in Chinese medicine industry, especially in manu- facturing process recently. Some of the experts use NIR to measure the content of Tanshinone and Salvianolic acid B in Danshen liquid distilling process [4] while some others use NIR in quantitative analysis of Gardenia herbs dis- tilling and Notoginsenosides [5-6]. NIR is also reported to be used in real-time analysis of CoptisRoot and Bit- tetOrange extract purification processes [7-8]. But these are limited in laboratory scale simulated manufacture research, but not yet in manufacture scale distilling or purification of TCM. This research covers the key working procedures in manufacturing of Salvianolic acid B, including distilling and purification, and focuses on concentration of Salvi- anolic acid B, which is the main effective ingredient in aqueous extract and elute. On-line NIRS chromatography charts are collected and chemometrical calculation is done based upon data from HPLC testing. Models for content analysis of Salvianolic acid B in distilling and purification processes are established in order to facilitate real-time quality monitoring in manufacturing of Salvi- anolic acid B. OBJECTIVE: