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Automatic Clustering of Flow Cytometry Data with Density-Based Merging英文文献资料.doc

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Automatic Clustering of Flow Cytometry Data with Density-Based Merging英文文献资料

HindawiPublishingCorporation AdvancesinBioinformatics Volume2009,ArticleID686759,7pages doi:10.1155/2009/686759 ResearchArticle AutomaticClusteringofFlowCytometryDatawith Density-BasedMerging GuentherWalther, StephenMeehan, 1 NoahZimmerman, 2 2 WayneMoore, 2 DavidParks, 2 2 2 IlanaBelitskaya, JinhuiPan, andLeonoreHerzenberg 2 1 2 DepartmentofStatistics,StanfordUniversity,Stanford,CA94305,USA DepartmentofGenetics,StanfordUniversity,Stanford,CA94305,USA CorrespondenceshouldbeaddressedtoGuentherWalther,gwalther@ Received1May2009;Revised27July2009;Accepted25August2009 RecommendedbyRaphaelGottardo Theabilityof?owcytometrytoallowfastsinglecellinterrogationofalargenumberofcellshasmadethistechnologyubiquitous andindispensableintheclinicalandlaboratorysetting.Acurrentlimittothepotentialofthistechnologyisthelackofautomated tools for analyzing the resulting data. We describe methodology and software to automatically identify cell populations in ?owcytometry data.Ourapproachadvancestheparadigmofmanuallygatingsequentialtwo-dimensionalprojectionsofthe data to a procedure that automatically produces gates based on statistical theory. Our approach is nonparametric and can reproducenonconvexsubpopulationsthatareknowntooccurin?owcytometrysamples,butwhichcannotbeproducedwith currentparametricmodel-basedapproaches.Weillustratethemethodologywithasampleofmousespleenandperitonealcavity cells. Copyright?2009GuentherWaltheretal. This is an open access article distributed under the Creative Commons Attribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. 1.Introduction describesubpopulationsofthemultivariate?owcytometry data. Flow cytometry allows to measure simultaneously multi- ple characteristics of thousands of cells. This ability has made ?ow cytometry a prevalent instrument in both the research and clinical settings. A major road block to tapping the full potential of this technology is the lack o