Bacterial Community Associated with Autotrophic and Heterotrophic Cultures of Medicinal Plant Smallanthus sonchifolius (Yacon)英文文献资料.docVIP

American Journal of Plant Sciences, 2012, 3, 1382-1389 /10.4236/ajps.2012.310167 Published Online October 2012 (http://www.SciRP.org/journal/ajps) Bacterial Community Associated with Autotrophic and Heterotrophic Cultures of Medicinal Plant Smallanthus sonchifolius (Yacon) Rita M. Moraes , Itamar S. Melo , Joko Sumyanto , Suman Chandra , Vaishali Joshi 1,2 3 1,2 1 1 1 National Center for Natural Products Research, Research Institute for Pharmaceutical Sciences, The University of Mississippi, Uni- versity, Oxford, USA; Center of Water and Wetland Resources, The University of Mississippi Field Station, Abbeville, USA; EMBRAPA/Environment, Rodovia SP 340 - Km 127.5, Caixa Postal 69, CEP, Jaguariúna SP, Brazil. Email: rmoraes@ 2 3 Received August 11 , 2012; revised September 8 , 2012; accepted September 18 , 2012 th th th ABSTRACT Several unfruitful attempts to grow axenic shoot cultures of Smallanthus sonchifolius, also known as yacon, were made before healthy shoots grew in association with bacteria on half strength Murashigue and Skoog media supplemented with 2.2 μM benzylaminopurine. Twenty-one bacterial isolates were obtained from in vitro S. sonchifolius plantlets, eight of these isolates were identified as Flavimonas oryzihabitans, Curtobacterium pusillum, Sphingomonas paucimo- bilis, and Microbacterium imperiale. These microorganisms produced indole acetic acid (IAA) at amounts varying be- tween 8.89 to 47.45 μg/mL, reason for being classified as plant growth promoting bacteria (PGPB). The results show that buds associated with bacteria cultured on sucrose free media produced 3.77 new roots mea