Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs英文文献资料.docVIP

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Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs英文文献资料.doc

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Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs英文文献资料

HindawiPublishingCorporation AdvancesinVirology Volume2011,ArticleID713769,11pages doi:10.1155/2011/713769 ResearchArticle BacterialToxinFusionProteinsElicitMucosal ImmunityagainstaFoot-and-MouthDiseaseVirusAntigen WhenAdministeredIntranasallytoGuineaPigs SreerupaChalla,1,2,3StevenM.Szczepanek,2,4DebraRood, 2,4 RogerW.Barrette,1,2,5andLawrenceK.Silbart 2,4 1 2 3 DepartmentofAnimalScience,UniversityofConnecticut,Storrs,CT06269,USA CenterofExcellenceforVaccineResearch,UniversityofConnecticut,Storrs,CT06269-2191,USA DepartmentofBiomedicalSciences,DivisionofInfectiousDiseases,TuftsCummingsSchoolofVeterinaryMedicine, NorthGrafton,MA01536,USA 4 5 DepartmentofAlliedHealthSciences,UniversityofConnecticut,Storrs,CT06269,USA PlumIslandAnimalDiseaseCenter,USDA-APHIS/VS/FADDL,Greenport,NY11944,USA CorrespondenceshouldbeaddressedtoLawrenceK.Silbart,lawrence.silbart@ Received15April2011;Revised7June2011;Accepted21June2011 AcademicEditor:GeorgeK.Lewis Copyright?2011SreerupaChallaetal. This is an open access article distributed under the Creative Commons Attribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. Peptidescorrespondingtothefoot-and-mouthdiseasevirusVP1G-Hlooparecapableofinducingneutralizingantibodiesin somespeciesbutareconsideredrelativelypoorimmunogens,especiallyatmucosalsurfaces.However,intranasaladministrationof antigensalongwiththeappropriatedeliveryvehicle/adjuvanthasbeenshowntoinducemucosalimmuneresponses,andbacterial enterotoxinshavelongbeenknowntobee?ectiveinthisregard.Inthecurrentstudy,twodi?erentcarrier/adjuvantapproaches wereusedtoaugmentmucosalimmunitytotheFMDVO1BFSG-Hloopepitope,inwhichtheG-Hloopwasgeneticallycoupledto theE.coliLT-BsubunitandcoexpressedwiththeLTA2fragment(LTA2B-GH),orthenontoxicpseudomonasexotoxinA(ntPE) wasfusedtoLTA2B-GHatLT-A2toenhancereceptortargeting.OnlyguineapigsthatwereinoculatedintranasallywithntPE- LTA2B-GHandLTA2B-GHinducedsigni?cantanti-G-HloopIgAantibo