Biomolecular Interaction Study of Cyclolinopeptide A with Human Serum Albumin英文文献资料.docVIP

HindawiPublishingCorporation JournalofBiomedicineandBiotechnology Volume2010,ArticleID737289,8pages doi:10.1155/2010/737289 ResearchArticle BiomolecularInteractionStudyofCyclolinopeptideAwith HumanSerumAlbumin BenRempel, BoGui, JasonMaley, MartinReaney, 1 2 3 4andRamaswamiSammynaiken 1,3 1 2 3 4 DepartmentofBiochemistry,UniversityofSaskatchewan,Saskatoon,SK,CanadaS7N5E5 DepartmentofFoodandBioproductSciences,UniversityofSaskatchewan,Saskatoon,SK,CanadaS7N5A8 SaskatchewanStructuralSciencesCentre,UniversityofSaskatchewan,Saskatoon,SK,CanadaS7N5C9 DepartmentPlantSciences,UniversityofSaskatchewan,Saskatoon,SK,CanadaS7N5A8 CorrespondenceshouldbeaddressedtoJasonMaley,j.maley@usask.caandRamaswamiSammynaiken,r.sammynaiken@usask.ca Received31August2010;Revised4December2010;Accepted21December2010 AcademicEditor:AbdelA.Abdel-Rahman Copyright?2010BenRempeletal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited. Thekinetics,energetics,andstructureofCyclolinopeptideAbindingwithHumanSerumAlbuminwereinvestigatedwithsurface plasmonresonanceandcirculardichroism.Thecomplexisformedthroughslowrecognitionkineticsthatistemperaturesensitive intherangeof20 C–37 ? ? C.Theoverallreactionwasobservedtobeendothermic(ΔH =204kJmol?1)andentropydriven(ΔS= 746Jmol?1K?1)withoverallsmallchangestothetertiarystructure. 1.Introduction T-lymphocytecelldivision[13],aswellasshowantimalarial activity[14]. Dependingonthepeptidesequence,CLPsmayhavean The nutritional bene?ts of Linum usitatissium seed, more commonly known as ?axseed, are of signi?cant interest as ?ax is seen as healthy [1]. Oil is the main constituent of ?axseed, with more than 70% of the oil composed of polyunsaturated fat. Flaxseed oil is the richest source of the essential omega-3 fatty acid, alpha-linolenic acid. Biotechnology and biomedicine around ?ax is devel