Catalytic Therapy of Cancer with Ascorbate and Extracts of Medicinal Herbs英文文献资料.docVIP

Advance Access Publication 26 December 2007 eCAM 2010;7(2)203–212 doi:10.1093/ecam/nem159 Original Article Catalytic Therapy of Cancer with Ascorbate and Extracts of Medicinal Herbs Nadejda Rozanova (Torshina)1, Jin Z. Zhang1 and Diane E. Heck2 1Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064 and 2Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine, Peoria, IL 61605, USA Catalytic therapy (CT) is a cancer treatment modality based on the generation of reactive oxygen species (ROS) using a combination of substrate molecules and a catalyst. The most frequently used substrate/catalyst pair is ascorbate/Co phthalocyanine (PcCo). In the present work, herb extracts containing pigments have been studied as a catalyst in place of PcCo. Extracts from herbs are expected to have efficiency comparable with that of phthalocyanines but as natural products, to exhibit fewer side effects. The present studies demonstrate that a combined use of ascorbate and herbal extracts results in ROS production and a significant decreaseinthenumberofcancercellsafterasingleinvitrotreatment.Treatmentwithascorbate in conjunction with extracts prepared from several medicinal herbs stimulated apoptosis and disrupted the cell cycle. The number of cells accumulating in the sub-G0/G1 stage of the cell cyclewasincreased2-to7-fold,andcellsinG2/Mincreased1.5-to20-fold,indicatingthatthe treatmentprotocolwashighlyeffectiveinsuppressingDNAsynthesisandpotentiallyreflecting DNA damage in the tumor cells. In addition, 20–40% of the cells underwent apoptosis within 24h of completing treatment. Our results suggest that herbal extracts can function as CT catalysts in the treatment of cancer. Introduction antitumor activity of this approach is the production of reactive oxygen species (ROS). Potentially, ROS gener- ated in response to these treatments interact with critical cellular moieties resulting inthe oxidative degradation of molec