C. trachomatis in Female Reproductive Tract Infections and RFLP-Based Genotyping A 16-Year Study from a Tertiary Care Hospital英文文献资料.docVIP

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C. trachomatis in Female Reproductive Tract Infections and RFLP-Based Genotyping A 16-Year Study from a Tertiary Care Hospital英文文献资料.doc

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C. trachomatis in Female Reproductive Tract Infections and RFLP-Based Genotyping A 16-Year Study from a Tertiary Care Hospital英文文献资料

HindawiPublishingCorporation InfectiousDiseasesinObstetricsandGynecology Volume2011,ArticleID548219,6pages doi:10.1155/2011/548219 ResearchArticle C.trachomatisinFemaleReproductive TractInfectionsandRFLP-BasedGenotyping: A16-YearStudyfromaTertiaryCareHospital SatpathyGita, 1 MittalSuneeta, SharmaAnjana, NayakNiranjan, 2 1 1 andR.M.Pandey 4 MohantySujata, 3 1 DepartmentofOcularMicrobiology,DrR.P.CentreforOphthalmicSciences,AllIndiaInstituteofMedicalSciences, AnsariNagar,NewDelhi110029,India 2 3 4 DepartmentofObstetricsandGynaecology,AllIndiaInstituteofMedicalSciences,AnsariNagar,NewDelhi110029,India StemCellFacility,AllIndiaInstituteofMedicalSciences,AnsariNagar,NewDelhi110029,India DepartmentofBiostatistics,AllIndiaInstituteofMedicalSciences,AnsariNagar,NewDelhi110029,India CorrespondenceshouldbeaddressedtoSatpathyGita,gita.satpathy@ Received29December2010;Revised6April2011;Accepted3May2011 AcademicEditor:LouiseHafner Copyright?2011SatpathyGitaetal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited. Presence of Chlamydia trachomatis in endocervix was determined in 2466 women attending a tertiary care hospital in New Delhi,Indiaoveraperiodof16years,usingamonoclonal-baseddirectimmuno?uorescenceassay,tissuecultureisolation,anda conventionalPCRassay.Chlamydiaantigencouldbedetectedin391outof2466(15.85%)ofpatientsstudied;in27.27%women withPID,16.74%womenwithcervicitis,16.03%womenwithinfertility,and12.06%womenwithadversepregnancyoutcomes, respectively.Therewasastatisticallysigni?cantdecreasingtrendinChlamydiaantigenpositivitybetweentheyears1994–1999and 2000–2004;theapparentdeclineinantigenpositivitybetweentheyears2000–2004and2005–2010wasnotstatisticallysigni?cant. AntigendetectionassaydetectedequalnumberofpositivesasthePCRassay;tissuecultureisolationdemonstratedlowerpositivity. Inafewrepresentativespecimen