后沟堡子酒酒曲优势细菌的初步分离与鉴定.pdfVIP

第 51 卷第 20 期 湖北农业科学 Vo l. 51 No.20 2012 年 10 月 Huhei Agricultural Sciences OC I.,2012 后沟堡子洒洒曲优势细菌的初步分离与鉴定 赵文婿，石 瑛，王瑞祥，单树花 (太原师范学院生物系，太原 030031 ) 摘要:从山西省晋中市榆J欠区后沟堡子滔滔命样品中初步分离纯化出 2 株细菌 XJl和 XJ2. 运过形态观 察以及生理生化反应分析得出 XJl白色、中等大小、圆形、茵落呈放射状 XJ2 呈乳白色、形状规则、表面 豆粉末状、较致密c 通过生理生化试验得出，两株细菌的~I 味试验、甲基红试验同呈阳性，两株细菌均能 利用葡萄糖和半手L糖，均不能利用þ.!糖，XJl革兰氏染色呈阳性，XJ2革兰氏染色呈阴性~参照《伯杰氏细 菌鉴定手册》可以得出这两株茵株分别是孔酸杆菌属和芽抱杆菌属c 关键询:堡子酒;大曲;细菌;分离 中图分类号:Q93-331 文献标识码:A 文章编号:0439-8114(2012)20-4604-03 Isolation and Preliminary Identification of Bacteria from Distiller s Yeast of Buzi Wine in Hougou ,Yuci ZHAO Wen-jing ,SHI Ying ,WANG Rui-xiang ,SHAN Shu-hua (Department of Biulogy , Taiyuan l\ormal University , Taiyuan 030031 , Shanxi. China) Abstract; Two bacteria , XJ1 and XJ2. were isolated from distiller s yeast of Buzi wine in Hougou , Yuci district , Jinzhong city , Shanxi province. According to shape observatio口， XJ1 was white , medium size , circular shape and with radial hypha; XJ2 was cream color , regular shape , compact and with dusty exterior. According to physiological biochemist町 expenment ， 由e 2 bacteria were positive to indole experiment and methy red experiment. Both of them could use glucose and galactose while could not use sucrose 嘈 XJl was positive to gram staining while XJ2 was negative. Based on Bergey s Manual of Sys- tematic Bacteriology , the two bacteria was prilimaryily identified as Lactobacillus sp. and Bacillus sp Key words; Buzi wine; distiller s yease; hacteria; isolation 利用谷物发芽时产生的酶将原料本身精化成糖分， 酒类是中国第一大类发酵食品，现在人们视高 再用酵母菌将糖分转变成酒精;