夹心ELISA法测定正常人血浆sDC-SIGN浓度及其意义初步探讨.pdfVIP

• 254 • 分子诊断与治疗杂志 2014 年7 月 第6 卷第4 期 J Mol. Diagn Ther , Jllly 20 14, YoL 6 No . 4 ·论 著· 夹心ELISA 法测定正常人血浆 sDC-SIGN 浓度及其 意义初步探讨 陈尚良 1 ，2 李慧i杰2 李延利 2 梁剑锋1 陆倚雯 l 陈政良 2食 { 摘要] 自的 测定正常人血浆 sDC-SIGN 浓度。 方法 对本室建立的夹心 EUSA 法检测 s DC-SIGN 体系进行灵敏度和重复性评估，并采用该体系对352 例健康志愿者外周血进行 sDc-srGN 浓 度测定。 结果 本室所建立的检测体系灵敏度可达6 ng/n世 ，批内变异. 10.4， %，批间变异 13. 8%; 352 例志际者检出 sDC- SIGN 的比例约 58%， 中位数 1. 18 ng/时，百分之 75 分位数 11.94 ng/削。 结论 室建立的体系可初步用于人血浆sDC- SIGN 浓度测定; 正常人血浆sDC- SIGN 浓度较低，总体呈正偏 本 态分布。 [ 关键词] 可溶性 DC-SIGN; ELlSA; 健康志愿者; 血浆 P reliminary study o f soluble DC -SIGN concentration by ELlSA in he althy volunteers l 2 2 l l H CHEN Shangl.iaug ,2 , Lf Hllij ie , LI Yangü , LlANG Jianfeng , LU Qianwen , CHEN Zheughang (1. Zhaoq ing Center Blood Statiou s , Gll augdong , Zhaoq ing 526020 , China ; 2. Departmeut of [mmunology , SOll thern Medical Un iversity , Gllangdong , Gllangzhou 510515 , Chi na ) [ABST RACT ] Obje ctive To detect the concenlratiou$ 0 1 solllble DC - SIGN in nonn al hll man plasma . Methods The sensitivity and repeatability experiments wcre tested respect ively for the spec ific sDC-SIGN ELlSA which was developed in Ollr laboratory , ald then 352 serllm sampl.es rrom healthy volllnteers wered detected for sDC-SIGN concentration by the ELISA , Re s ults The sensitivity of ELISA developed in Ollr laboratory was up to 6 ng/n让 and the variation of group and inter batch were 10.4% and 13.8% , respωtively ， About 58% healthy donors contained detectable amounts of sDC-SIGN ranging froll1 0 to 187 ng/ ml with a medjan value of 1.18 ng/ [r让 Conclusions The specific sDC-SIGN ELISA wa