Analysis of Memory B Cell Responses and Isolation of Novel Monoclonal Antibodies with Neutralizing Breadth from HIV-1-Infected Individuals 英文参考文献.docVIP

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Analysis of Memory B Cell Responses and Isolation of Novel Monoclonal Antibodies with Neutralizing Breadth from HIV-1-Infected Individuals 英文参考文献.doc

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Analysis of Memory B Cell Responses and Isolation of Novel Monoclonal Antibodies with Neutralizing Breadth from HIV-1-Infected Individuals 英文参考文献

AnalysisofMemoryBCellResponsesandIsolationof NovelMonoclonalAntibodieswithNeutralizingBreadth fromHIV-1-InfectedIndividuals DavideCorti1,JohannesP.M.Langedijk2,AndreasHinz3,MichaelS.Seaman4,FabriziaVanzetta1, BlancaM.Fernandez-Rodriguez1,ChiaraSilacci1,DeboraPinna1,DavidJarrossay1 ,Sunita Balla-Jhagjhoorsingh5,BettyWillems5,MariaJ.Zekveld2,HannaDreja6,EithneO’Sullivan6 ,Corinna Pade6,ChloeOrkin7,SimonA.Jeffs8,DavidC.Montefiori9,DavidDavis10,WinfriedWeissenhorn3,Aine′ McKnight6,JonathanL.Heeney11,FedericaSallusto1,QuentinJ.Sattentau12,RobinA.Weiss13* ,Antonio Lanzavecchia1* 1InstituteforResearchinBiomedicine,Bellinzona,Switzerland,2PepscanTherapeuticsBV,Lelystad,Netherlands,3UnitforVirusHostCellInteraction,UMI3265UJF- EMBL-CNRS, Grenoble, France, 4Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States of America, 5Institute of TropicalMedicine,Antwerp,Belgium,6QueenMaryUniversity,London,UnitedKingdom,7BartsandtheLondonNHSTrust,London,UnitedKingdom,8Departmentof Infectious Diseases, The Wright-Fleming Institute, Imperial College Faculty of Medicine, London, United Kingdom, 9Duke University Medical Center, Durham, North Carolina, United States of America, 10Department of Virology, Biomedical Primate Research Centre, Rijswijk, Netherlands, 11Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom, 12The Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom, 13Division of InfectionandImmunity,UniversityCollegeLondon,London,UnitedKingdom Abstract Background: The isolation of human monoclonal antibodies (mAbs) that neutralize a broad spectrum of primary HIV-1 isolatesandthecharacterizationofthehumanneutralizingantibodyBcellresponsetoHIV-1infectionareimportantgoals thatarecentraltothedesignofaneffectiveantibody-basedvaccine. MethodsandFindings:WeimmortalizedIgG+memoryBcellsfromindividualsinfectedwithdiversecladesofHIV-1and selected on the basis of plasma neutrali