α-酮戊二酸修饰壳聚糖微球对牛血清蛋白的吸附.pdfVIP

学兔兔 化 工 进 展 2008年第27卷第12期 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS ·1991· ． 酮戊二酸修饰壳聚糖微球对牛血清蛋白的吸附 邓 俊，李明春，辛梅华，葛亚芳 (华侨大学材料科学与工程学院，福建省高校功能材料重点实验室，福建 泉州362021) 摘 要：采用反相悬浮法制备交联壳聚糖微球，再与a一酮戊二酸反应生成Schiff碱，NaBH4还原制得改性壳聚糖 微球。用FTm、SEM和XRD对其进行表征，并将之用于牛血清白蛋白的吸附研究。考察了吸附时间、溶液pH 值、牛血清白蛋白的浓度、温度、NaC1含量等因素对牛血清白蛋白吸附的影响。结果表明， 一酮戊二酸改性交联 壳聚糖微球不溶于酸和碱，对牛血清白蛋白具有良好的吸附性能，在pH=5．0时，吸附在1 h内可达平衡，吸附 数据符合Langmuir等温方程和Lagergren二级动力学方程。 关键词：壳聚糖； 一酮戊二酸；微球；吸附 中图分类号：O 636．1 文献标识码：A 文章编号：1000—6613(2008)12—1991—05 Adsorption of bovine serun albumin on chitosan microspheres modified by -ketoglutaric acid DENG Jun，LIMingchun，XINMeihua，GE Yafang (College of Material Science and Engineering，Huaqiao University，Key Laboratory of Functional Materials for Fujian Higher Education，Quanzhou 362021，Fujian，China) Abstract：Chitosan microspheres were prepared by the inverse phase suspension method．The chitosan was modified by reducing Schiff’S bases formed from the reaction of a—ketoglutaric acid and chitosan． The structure of microspheres was characterized bv FTIR，SEM and XRD．The effects of adsorption time，pH，temperature，concentrations of albumin bovine and sodium chloride on the adsorption efficiency of albumin bovine were studied．Th e experimental results indicated that microspheres were insoluble in acidic and basic solutions．The modified chitosan microspheres adsorbed albumin bovine very well and at pH 5．0 adsorption reached equilibrium in 1 h．The adsorption data was fitted to the Langmuir relationship and the Lagergren second—order adsorption kinetics．