Electroendocytosis Is Driven by the Binding of Electrochemically Produced Protons to the Cell’s Surface 英文参考文献.docVIP

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Electroendocytosis Is Driven by the Binding of Electrochemically Produced Protons to the Cell’s Surface 英文参考文献.doc

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Electroendocytosis Is Driven by the Binding of Electrochemically Produced Protons to the Cell’s Surface 英文参考文献

ElectroendocytosisIsDrivenbytheBindingof ElectrochemicallyProducedProtonstotheCell’sSurface NadavBen-Dov,InnaRozmanGrinberg,RafiKorenstein* DepartmentofPhysiologyandPharmacology,FacultyofMedicine,Tel-AvivUniversity,Tel-Aviv,Israel Abstract Electroendocytosisinvolvestheexposureofcellstopulsedlowelectricfieldandisemergingasacomplementarymethod toelectroporationfortheincorporationofmacromoleculesintocells.Thepresentstudyexplorestheunderlyingmechanism of electroendocytosis and its dependence on electrochemical byproducts formed at the electrode interface. Cell suspensionswereexposedtopulsedlowelectricfieldinapartitioneddevicewherecellsarespatiallyrestrictedrelativeto theelectrodes.Thecellularuptakeofdextran-FITCwasanalyzedbyflowcytometeryandvisualizedbyconfocalmicroscopy. We first show that uptake occurs only in cells adjacent to the anode. The enhanced uptake near the anode is found to dependonelectriccurrentdensity ratherthanonelectricfieldstrength,intherangeof5to65V/cm.Electrochemically produced oxidative species that impose intracellular oxidative stress, do not play any role in the stimulated uptake. An inversedependenceisfoundbetweenelectricallyinduceduptakeandthesolution’sbuffercapacity.Electroendocytosiscan bemimickedbychemicallyacidifyingtheextracellularsolutionwhichpromotestheenhanceduptakeofdextranpolymers andtheuptakeofplasmidDNA.Electrochemicalproductionofprotonsattheanodeinterfaceisresponsibleforinducing uptakeofmacromoleculesintocellsexposedtoapulsedlowelectricfield.Expandingtheunderstandingofthemechanism involvedinelectricfieldsinduceddrug-deliveryintocells,isexpectedtocontributetoclinicaltherapyapplicationsinthe future. Citation:Ben-DovN,RozmanGrinbergI,KorensteinR(2012)ElectroendocytosisIsDrivenbytheBindingofElectrochemicallyProducedProtonstotheCell’s Surface.PLoSONE7(11):e50299.doi:10.1371/journal.pone.0050299 Editor:AnnaMariaDelprato,BioScienceProject,UnitedStatesofAmerica ReceivedSeptember9,2012;AcceptedOctober18,2012;PublishedNovember27,2012 C