ER-α36, a Novel Variant of ER-α, Mediates Estrogen-Stimulated Proliferation of Endometrial Carcinoma Cells via the PKCδERK Pathway 英文参考文献.docVIP

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ER-α36, a Novel Variant of ER-α, Mediates Estrogen-Stimulated Proliferation of Endometrial Carcinoma Cells via the PKCδERK Pathway 英文参考文献.doc

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ER-α36, a Novel Variant of ER-α, Mediates Estrogen-Stimulated Proliferation of Endometrial Carcinoma Cells via the PKCδERK Pathway 英文参考文献

ER-a36,aNovelVariantofER-a,MediatesEstrogen- StimulatedProliferationofEndometrialCarcinomaCells viathePKCd/ERKPathway Jing-ShanTong1,2,Qing-HuaZhang2,Zhen-BoWang2,SenLi2,Cai-RongYang2,Xue-QiFu1,YiHou2, Zhao-YiWang3,JunSheng1,4*,Qing-YuanSun2* 1CollegeofLifeSciences,JilinUniversity,Changchun,China,2StateKeyLaboratoryofReproductiveBiology,InstituteofZoology,ChineseAcademyofSciences,Beijing, China,3DepartmentofMedicalMicrobiologyandImmunology,CreightonUniversityMedicalSchool,Omaha,Nebraska,UnitedStatesofAmerica,4YunnanAgricultural University,Kunming,China Abstract Background:Recently,avariantofER-a,ER-a36wasidentifiedandcloned.ER-a36lacksintrinsictranscriptionactivityand mainly mediates non-genomic estrogen signaling. The purpose of this study was to investigate the function and the underlyingmechanismsofER-a36ingrowthregulationofendometrialIshikawacancercells. Methods: The cellular localization of ER-a36 and ER-a66 were determined by immunofluorescence in the Ishikawa cells. Ishikawa endometrial cancer control cells transfected with an empty expression vector, Ishikawa cells with shRNA knockdownofER-a36(Ishikawa/RNAiER36)andIshikawacellswithshRNAknockdownofER-a66(Ishikawa/RNAiER66)were treated with E2 and E2-conjugated to bovine serum albumin (E2-BSA, membrane impermeable) in the absence and presenceofdifferentkinaseinhibitorsHBDDE,bisindolylmaleimide,rottlerin,H89andU0126.Thephosphorylationlevelsof signaling molecules and cyclin D1/cdk4 expression were examined with Western blot analysis and cell growth was monitoredwiththeMTTassay. Results: Immunofluorescence staining of Ishikawa cells demonstrated that ER-a36 was expressed mainly on the plasma membraneandinthecytoplasm,whileER-a66waspredominantlylocalizedinthecellnucleus.BothE2andE2-BSArapidly activatedPKCdnotPKCainIshikawacells,whichcouldbeabrogatedbyER-a36shRNAexpression.E2-andE2-BSA-induced ERKphosphorylationrequiredER-a36andPKCd.However,onlyE2wasabletoinduceCamp-dependentproteinkinaseA (PKA)phosphorylation.Fu