Eukaryotic Translation Elongation Factor 1A (eEF1A) Domain I from S. cerevisiae Is Required but Not Sufficient for Inter-Species Complementation 英文参考文献.docVIP
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Eukaryotic Translation Elongation Factor 1A (eEF1A) Domain I from S. cerevisiae Is Required but Not Sufficient for Inter-Species Complementation 英文参考文献
EukaryoticTranslationElongationFactor1A(eEF1A)
DomainIfromS.cerevisiaeIsRequiredbutNotSufficient
forInter-SpeciesComplementation
SandraEltschinger1.,EvaGreganova1,2.,ManfredHeller3,PeterBu¨tikofer1*,MichaelAltmann1*
1Institute of Biochemistry Molecular Medicine, University of Bern, Bern, Switzerland, 2Swiss Tropical and Public Health Institute, Basel, Switzerland, 3Mass
SpectrometryandProteomics,DepartmentofClinicalResearch,UniversityHospital,Bern,Switzerland
Abstract
Ethanolamine phosphoglycerol (EPG) is a protein modification attached exclusively to eukaryotic elongation factor 1A
(eEF1A).Inmammalsandplants,EPGislinkedtoconservedglutamateresidueslocatedineEF1AdomainsIIandIII,whereas
intheunicellulareukaryoteTrypanosomabrucei,onlydomainIIIismodifiedbyasingleEPG.AbiosyntheticprecursorofEPG
andstructuralrequirementsforEPGattachmenttoT.bruceieEF1Ahavebeenreported,butnothingisknownabouttheEPG
modifyingenzyme(s).ByexpressinghumaneEF1AinT.brucei,wenowshowthatEPGattachmenttoeEF1Aisevolutionarily
conservedbetweenT.bruceiandHomosapiens.Incontrast,S.cerevisiaeeEF1A,whichhasbeenshowntolackEPGisnot
modifiedinT.brucei.Furthermore,weshowthateEF1AcannotfunctionallycomplementacrossspecieswhenusingT.brucei
andS.cerevisiaeasmodelorganisms.However,functionalcomplementationinyeastcanbeobtainedusingeEF1Achimera
containingdomainsIIorIIIfromotherspecies.Incontrast,yeastdomainIisstrictlyrequiredforfunctionalcomplementation
inS.cerevisiae.
Citation:EltschingerS,GreganovaE,HellerM,Bu¨tikoferP,AltmannM(2012)EukaryoticTranslationElongationFactor1A(eEF1A)DomainIfromS.cerevisiaeIs
RequiredbutNotSufficientforInter-SpeciesComplementation.PLoSONE7(7):e42338.doi:10.1371/journal.pone.0042338
Editor:ThomasPreiss,TheJohnCurtinSchoolofMedicalResearch,Australia
ReceivedApril27,2012;AcceptedJuly3,2012;PublishedJuly30,2012
Copyright: ?2012 Eltschinger etal.Thisisanopen-access article distributedunder thetermsoftheCreativeCommonsAttributionLicense,which permits
unrestricteduse,distribution,andrepr
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