Evaluation of MCM-2 Expression in TMA Cervical Specimens 英文参考文献.docVIP

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Evaluation of MCM-2 Expression in TMA Cervical Specimens 英文参考文献.doc

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Evaluation of MCM-2 Expression in TMA Cervical Specimens 英文参考文献

EvaluationofMCM-2ExpressioninTMACervical Specimens AlcinaF.Nicol1*,Jose′ R.LapaeSilva2,CynthiaB.Cunha3,SergioM.Amaro-Filho1,NathaliaOliveira1, BeatrizGrinsztejn3,RuthKhalil3,FabioRussomano4,AndreaPires5,JonathanE.Golub6,GerardJ.Nuovo7 1LaboratoryInterdisciplinaryofMedicalResearch,InstitutoOswaldoCruz,RiodeJaneiro,Brazil,2Laborato′rioMultidisciplinar,ClementinoFragaFilhoUniversityHospital, UniversidadeFederaldoRiodeJaneiro,RiodeJaneiro,Brazil,3DepartmentofInfectiousDisease,EvandroChagasClinicalResearchInstitute,Fundac?a?oOswaldoCruz,Rio de Janeiro, Brazil, 4Department of Cervical Pathology, Fernandes Figueira Institute, Fundac?a?o Oswaldo Cruz, Rio de Janeiro, Brazil, 5Fonte Medicina Diagnostic LaboratoryandFederalFluminenseUniversity,Nitero′i,RiodeJaneiro,Brazil,6JohnsHopkinsBloombergSchoolofPublicHealthandJohnsHopkinsSchoolofMedicine, Baltimore,Maryland,UnitedStatesofAmerica,7ComprehensiveCancerCenter,OhioStateUniversity,Columbus,Ohio,UnitedStatesofAmerica Abstract Background:Minichromosomemaintenanceproteins(MCM)arehighlyexpressedinactivelyreplicatingcells.Theneedfor biological markers for cervical carcinoma and its precursor lesions is emerging. Our main aim was to determine the immunohistochemicalexpressionofMCM-2inHIV-positiveand-negativedysplasticcervicalspecimens. Methods: Immunohistochemical analysis of MCM-2 was performed in a total of 352 cervical TMA specimens of normal control, low-grade CIN, high-grade CIN and invasive tumor. 38 specimens were from HIV-positive women. A receiver operatingcharacteristic(ROC)curvewasconstructedtodeterminethebestcutofftodiagnosehigh-gradeCINandinvasive cervicalcancer. Results:Intheprogressionfromnormalepitheliumtohigh-gradeCINandinvasivetumorwefoundsignificantdifferences intheMCM-2expression(p,0.05).BasedontheROCcurveof80%withanareaunderthecurve(AUC)of0.78,expressionof MCM-2todiagnosehigh-gradeCINandinvasivetumorresultedinsensitivityof81%,specificityof66%,apositivepredictive value(PPV)of86%andanegativepredictivevalue(NP