Evidence on the Formation of Singlet Oxygen in the Donor Side Photoinhibition of Photosystem II EPR Spin-Trapping Study 英文参考文献.docVIP

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Evidence on the Formation of Singlet Oxygen in the Donor Side Photoinhibition of Photosystem II EPR Spin-Trapping Study 英文参考文献.doc

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Evidence on the Formation of Singlet Oxygen in the Donor Side Photoinhibition of Photosystem II EPR Spin-Trapping Study 英文参考文献

EvidenceontheFormationofSingletOxygeninthe DonorSidePhotoinhibitionofPhotosystemII:EPRSpin- TrappingStudy DeepakKumarYadav,PavelPosp?′sˇil* DepartmentofBiophysics,CentreoftheRegionHana′ forBiotechnologicalandAgriculturalResearch,FacultyofScience,Palacky′ University,Olomouc,CzechRepublic Abstract WhenphotosystemII(PSII)isexposedtoexcesslight,singletoxygen(1O2)formedbytheinteractionofmolecularoxygen N+ N2 withtripletchlorophyll.Tripletchlorophyllisformedbythechargerecombinationoftripletradicalpair 3[P680 Pheo ]in the acceptor-side photoinhibition of PSII. Here, we provide evidence on the formation of 1O2 in the donor side photoinhibitionofPSII.Light-induced 1O2productioninTris-treatedPSIImembraneswasstudiedbyelectronparamagnetic resonance (EPR) spin-trapping spectroscopy, as monitored by TEMPONE EPR signal. Light-induced formation of carbon- N centered radicals (R) was observed by POBN-R adduct EPR signal. Increased oxidation of organic molecules at high pH enhanced the formation of TEMPONE and POBN-R adduct EPR signals in Tris-treated PSII membranes. Interestingly, the scavenging of R by propyl gallate significantly suppressed 1O2. Based on our results, it is concluded that 1O2 formation correlateswithR formationonthedonorsideofPSIIduetooxidationoforganicmolecules(lipidsandproteins)bylong- N N N+ N livedP680 /TyrZ.ItisproposedherethattheRussellmechanismfortherecombinationoftwoperoxylradicalsformedby theinteractionofR withmolecularoxygenisaplausiblemechanismfor1O2formationinthedonorsidephotoinhibitionof PSII. N Citation: Yadav DK, Posp?′sˇil P (2012) Evidence on the Formation of Singlet Oxygen in the Donor Side Photoinhibition of Photosystem II: EPR Spin-Trapping Study.PLoSONE7(9):e45883.doi:10.1371/journal.pone.0045883 Editor:MariaGasset,ConsejoSuperiordeInvestigacionesCientificas,Spain ReceivedMay15,2012;AcceptedAugust27,2012;PublishedSeptember26,2012 Copyright: ? 2012 Yadav, Posp?′sˇil. This is an open-access article distributed under the te