Evidence that Plasmid-Borne Botulinum Neurotoxin Type B Genes Are Widespread among Clostridium botulinum Serotype B Strains 英文参考文献.docVIP

下载本文档

5
0
约4.28万字
约 9页
2017-05-11 发布于上海
举报
版权申诉

Evidence that Plasmid-Borne Botulinum Neurotoxin Type B Genes Are Widespread among Clostridium botulinum Serotype B Strains 英文参考文献.doc

1、本文档共9页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Evidence that Plasmid-Borne Botulinum Neurotoxin Type B Genes Are Widespread among Clostridium botulinum Serotype B Strains 英文参考文献

EvidencethatPlasmid-BorneBotulinumNeurotoxinType BGenesAreWidespreadamongClostridiumbotulinum SerotypeBStrains GiovannaFranciosa*,AntonellaMaugliani,ConcettaScalfaro,PaoloAureli DepartmentofFoodSafetyandVeterinaryPublicHealth,UnitofMicroorganismsandFoodTechnologies,IstitutoSuperiorediSanita`, Rome,Italy Abstract Background:PlasmidsthatencodecertainsubtypesofthebotulinumneurotoxintypeBhaverecentlybeendetectedin someClostridiumbotulinumstrains.Theobjectiveofthepresentstudywastoinvestigatethefrequencywithwhichplasmid carriage of the botulinum neurotoxin type B gene (bont/B) occurs in strains of C. botulinum type B, Ab, and A(B), and whetherplasmidcarriageisbont/Bsubtype-related. Methodology/PrincipalFindings:PCR-Restrictionfragmentlengthpolymorphismwasemployedtoidentifysubtypesofthe bont/B gene. Pulsed-field gel electrophoresis and Southern blot hybridization with specific probes were performed to analyzethegenomiclocationofthebont/Bsubtypegenes.Allfiveknownbont/Bsubtypegenesweredetectedamongthe strains; the most frequently detected subtype genes were bont/B1 and /B2. Surprisingly, the bont/B subtype gene was shown to be plasmid-borne in .50% of the total strains. The same bont/B subtype gene was associated with the chromosomeinsomestrains,whereasitwasassociatedwithaplasmidinothers.Allfiveknownbont/Bsubtypegeneswere insomecasesfoundtoresideonplasmids,thoughwithvaryingfrequency(e.g.,mostofthebont/B1subtypegeneswere located on plasmids, whereas all but one of the bont/B2 subtypes were chromosomally-located). Three bivalent isolates carried both bont/A and /B genes on the same plasmid. The plasmids carrying the bont gene were five different sizes, rangingfrom,55kbto,245kb. Conclusions/Significance:Theunexpectedfindingofthewidespreaddistributionofplasmidsharboringthebont/Bgene amongC.botulinumserotypeBstrainsprovidesachancetoexaminetheircontributiontothedisseminationofthebont genesamongheterogeneousclostridia,withpotentialimplicationsonissuesrelatedtopathogenesi