Expression Analysis of the NLRP Gene Family Suggests a Role in Human Preimplantation Development 英文参考文献.docVIP

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Expression Analysis of the NLRP Gene Family Suggests a Role in Human Preimplantation Development 英文参考文献.doc

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Expression Analysis of the NLRP Gene Family Suggests a Role in Human Preimplantation Development 英文参考文献

ExpressionAnalysisoftheNLRPGeneFamilySuggestsa RoleinHumanPreimplantationDevelopment PuZhang1,MoragDixon1,MarcoZucchelli1,FredwellHambiliki2,3,LevLevkov2,OutiHovatta2. ,Juha Kere1. * 1DepartmentofBioscienceandNutritionatNovum,KarolinskaInstitutet,Huddinge,Sweden,2DepartmentofClinicalScience,InterventionandTechnology,Divisionof ObstetricsandGynecology,K57,KarolinskaUniversityHospitalHuddinge,Stockholm,Sweden,3CentreforReproduction,DepartmentofGynecology,UppsalaUniversity Hospital,Uppsala,Sweden Abstract Background: The NLRP (Nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain containing) family,alsoreferredtoasNALPfamily,iswellknownforitsrolesinapoptosisandinflammation.SeveralNLRPshavebeen indicatedasbeinginvolvedinreproductionaswell. Methodology:Westudied,usingtheuniquehumangametesandembryomaterials,theexpressionoftheNLRPfamilyin human gametes and preimplantation embryos at different developmental stages, and compared the expression levels betweennormalandabnormalembryosusingreal-timePCR. PrincipalFindings:Among14membersoftheNLRPfamily,twelveweredetectedinhumanoocytesandpreimplantation embryos,whereassevenweredetectedinspermatozoa.EightNLRPs(NLRP4,5,8,9,11,12,13,and14)showedasimilar expressionpattern:theirexpressionlevelswerehighinoocytesandthendecreasedprogressivelyinembryos,resultingina very low level in day 5 embryos. However, NLRP2 and NLRP7 showed a different expression pattern: their expression decreasedfromoocytestothelowestlevelbyday3,butincreasedagainbyday5.TheexpressionlevelsofNLRP5,9,and12 were lower in day 1 abnormal embryos but higher in day3 and day5 arrested embryos, when compared with normal embryosatthesamestages.NLRP7wasdown-regulatedinday1andday5abnormalembryosbutover-expressedinday3 arrestedembryos. Conclusions: According to our results, different NLRPs possibly work in a stage-dependent manner during human preimplantationdevelopment. Citation: Zhang P, Dixon M, Zucchelli M, Hambiliki F, Levkov L, et al. (2008) Expre