Expression and In Situ Localization of Two Major PR Proteins of Grapevine Berries during Development and after UV-C Exposition 英文参考文献.docVIP

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Expression and In Situ Localization of Two Major PR Proteins of Grapevine Berries during Development and after UV-C Exposition 英文参考文献.doc

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Expression and In Situ Localization of Two Major PR Proteins of Grapevine Berries during Development and after UV-C Exposition 英文参考文献

ExpressionandInSituLocalizationofTwoMajorPR ProteinsofGrapevineBerriesduringDevelopmentand afterUV-CExposition StevenColas,DamienAfoufa-Bastien,LucileJacquens,ChristopheCle′ment,FabienneBaillieul, FlorenceMazeyrat-Gourbeyre*.,LaurenceMonti-Dedieu. LaboratoiredeStress,De′fensesetReproductiondesPlantes,Unite′ deRechercheVignesetVinsdeChampagne-EA4707,UFRSciencesExactesetNaturelles,Universite′ deReimsChampagne-Ardenne,BP1039,Reims,France Abstract In grapevine Vitis vinifera L. cv Pinot noir, the Pathogenesis-Related (PR) proteins CHI4D and TL3 are among the most abundantextractablePRproteinsofripeberriesandaccumulateduringberryripeningfromve′raison untilfullmaturation. Evidencewassuppliedinfavoroftheinvolvementofthesetwoproteinfamiliesinplantdefensemechanismsandplant development.InordertobetterunderstandCHI4DandTL3functioningrapevine,weanalyzedtheirtemporalandspatial pattern of expression during maturation and after an abiotic stress (UV-C) by in situ hybridization (ISH) and immunohistolocalization. In ripening berries, CHI4D and TL3 genes were mainly expressed in the exocarp and around vascularbundlesofthemesocarp.InUV-Cexposedberries,CHI4DandTL3geneexpressionwasstronglyinducedbefore ve′raison.Correspondingproteinslocalizedintheexocarpand,toalesserextent,aroundvascularbundlesofthemesocarp. The spatial and temporal accumulation of the two PR proteins during berry maturation and after an abiotic stress is discussedinrelationtotheirputativerolesinplantdefense. Citation:ColasS,Afoufa-BastienD,JacquensL,Cle′mentC,BaillieulF,etal.(2012)ExpressionandInSituLocalizationofTwoMajorPRProteinsofGrapevine BerriesduringDevelopmentandafterUV-CExposition.PLoSONE7(8):e43681.doi:10.1371/journal.pone.0043681 Editor:MariaGasset,ConsejoSuperiordeInvestigacionesCientificas,Spain ReceivedFebruary27,2012;AcceptedJuly24,2012;PublishedAugust24,2012 Copyright: ? 2012 Colas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License,