First Transcriptome of the Testis-Vas Deferens-Male Accessory Gland and Proteome of the Spermatophore from Dermacentor variabilis (Acari Ixodidae) 英文参考文献.docVIP

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First Transcriptome of the Testis-Vas Deferens-Male Accessory Gland and Proteome of the Spermatophore from Dermacentor variabilis (Acari Ixodidae) 英文参考文献.doc

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First Transcriptome of the Testis-Vas Deferens-Male Accessory Gland and Proteome of the Spermatophore from Dermacentor variabilis (Acari Ixodidae) 英文参考文献

FirstTranscriptomeoftheTestis-VasDeferens-Male AccessoryGlandandProteomeoftheSpermatophore fromDermacentorvariabilis(Acari:Ixodidae) DanielE.Sonenshine1*,BrookeW.Bissinger2,NobleEgekwu1,KevinV.Donohue2,SayedM.Khalil2 ,R. MichaelRoe2 1DepartmentofBiologicalSciences,OldDominionUniversity,Norfolk,Virginia,UnitedStatesofAmerica,2DepartmentofEntomology,NorthCarolinaStateUniversity, Raleigh,NorthCarolina,UnitedStatesofAmerica Abstract Ticksareimportantvectorsofnumeroushumandiseasesandanimaldiseases.Feedingstimulatesspermatogenesis,mating andinseminationofmalefactorsthattriggerfemalereproduction.Thephysiologyofmalereproductionanditsregulation offemaledevelopmentareessentiallyablackbox.Severaltranscriptomeshavecataloguedexpressionoftickgenesinthe salivaryglands,synganglionandmidgutbutnocomprehensiveinvestigationhasaddressedmalereproductionandmating. Consequently, a new global approach using transcriptomics, proteomics, and quantitative gene expression is needed to understandmalereproductionandstimulationoffemalereproduction. Thisfirsttranscriptometothereproductivebiology of fed male ticks, Dermacentor variabilis, was obtained by 454 pyrosequencing (563,093 reads, 12,804 contigs). Gene Ontology (Biological Processes level III) recognized 3,866 transcripts in 73 different categories; spermiogenesis; spermatogenesis; peptidases, lipases and hydrolases; oxidative and environmental stress; immune defense; and protein binding. Reproduction-associated genes included serine/threonine kinase, metalloendoproteinases, ferritins, serine proteases, trypsin, cysteine proteases, serpins, a cystatin, GPCR and others. qRT-PCR showed significant upregulation fromunfedversusfedadultmalereproductiveorgansofzincmetalloprotease,astacinmetalloproteaseandserineprotease, enzymes important in spermiogenesis and mating activity in insects, as well as a GPCR with the greatest similarity to a SIFamidereceptorknowntobeimportantinregulatingcourtshipbehaviorinDrosophila.Proteomicsontheseorgansand