Functional and Probiotic Attributes of an Indigenous Isolate of Lactobacillus plantarum 英文参考文献.docVIP

FunctionalandProbioticAttributesofanIndigenous IsolateofLactobacillusplantarum JaiK.Kaushik1*,AshutoshKumar2¤,RajK.Duary2,AshokK.Mohanty1,SunitaGrover2,VirenderK. Batish2* 1AnimalBiotechnologyCentre,NationalDairyResearchInstitute,Karnal,India,2MolecularBiologyUnit,DairyMicrobiologyDivision,NationalDairyResearchInstitute, Karnal,India Abstract Background: Probiotic microorganisms favorably alter the intestinal microflora balance, promote intestinal integrity and mobility, inhibit the growth of harmful bacteria and increase resistance to infection. Probiotics are increasingly used in nutraceuticals,functionalfoodsorinmicrobialinterferencetreatment.However,theeffectivenessofprobioticorganismis consideredtobepopulation-specificduetovariationingutmicroflora,foodhabitsandspecifichost-microbialinteractions. MostoftheprobioticstrainsavailableinthemarketareofwesternorEuropeanorigin,andastrongneedforexploringnew indigenousprobioticorganismsisfelt. Methods and Findings: An indigenous isolateLp9 identified as Lactobacillus plantarum by molecular-typing methodswas studied extensively for its functional and probiotic attributes, viz., acid and bile salt tolerance, cell surface hydrophobicity, autoaggregation and Caco-2 cell-binding as well as antibacterial and antioxidative activities. Lp9 isolate could survive 2h incubation at pH1.5–2.0 and toxicity of 1.5–2.0% oxgall bile. Lp9 could deconjugate major bile salts like glycocholate and deoxytaurocholate,indicatingitspotentialtocausehypocholesterolemia.Theisolateexhibitedcell-surfacehydrophobicityof ,37% and autoaggregation of ,31%. Presence of putative probiotic marker genes like mucus-binding protein (mub), fibronectin-bindingprotein(fbp)andbilesalthydrolase(bsh)wereconfirmedbyPCR.Presenceofthesegenessuggestedthe possibilityof specific interaction and colonization potentialof Lp9 isolate in the gut, which was also suggested by a good adhesionratioof7.461.3%withCaco-2cellline.Theisolatedemonstratedhigherfreeradicalscavengingacti